https://ri.ufs.br/jspui/handle/riufs/113 2026-10-28T17:36:41Z https://ri.ufs.br/jspui/handle/riufs/26111 Title: Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken Authors: Oliveira, Haniel Cedraz de; Garcia Junior, Antonio Amandio Pinto; Gromboni, Juliana Gracielle Gonzaga; Farias Filho, Ronaldo Vasconcelos; Nascimento, Carlos Souza do; Wenceslau, Amauri Arias Abstract: Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary. The best normalizer is that gene which remains stable in all samples from different treatments. The aim of this study was to identify stable reference genes for normalization of target genes in muscle tissue from three genetically divergent chickens groups (Peloco, Cobb 500® and Caneluda) under environmental (heat stress and comfort) and sex influence. Expressions of ten reference genes were tested for stability in breast muscular tissue (Pectoralis major muscle). Samples were obtained from 36 males and females of two backyard breeds (Caneluda and Peloco) and one commercial line (Cobb 500®) under two environments. The heat stress and comfort temperature were 39 and 23˚C, respectively. Animals were housed in the Animal Science Department at Universidade Estadual do Sudoeste da Bahia. We analyzed the expression data by four statistical tools (SLqPCR, NormFinder, Bestkeeper and Comparative CT). According to these tools, genes stability varied according to sex, genetic group and environment, however, some genes remained stable in all analyzes. There was no difference between the most stable genes for sex effect, being MRPS27 more stable for both males and females. In general, MRPS27 was the most stable gene. Within the three genetic groups, the most stable genes were RPL5, HMBS and EEF1 to Cobb 500®, Peloco and Caneluda, respectively. Within the environment, the most stable gene under comfort and heat stress conditions was HMBS and MRPS27, respectively. BestKeeper and Comparative Ct were less correlated (28%) and SLqPCR and NormFinder were the most correlated (98%). MRPS27, RPL5 and MRPS30 genes were considered stable according the overall ranking and can be used as normalizer of relative expression of target genes in muscle tissue of chickens under heat stress. 2017-01-01T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/25643 Title: Quality of life and gait in elderly group Authors: Taguchi, Carlos Kazuo; Teixeira, Jacqueline Pitanga; Alves, Lucas Vieira; Oliveira, Priscila Feliciano; Raposo, Oscar Felipe Falcão 2016-01-01T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/25597 Title: Autoavaliação da comunicação em promotores de justiça em um estado do nordeste, Brasil Authors: Sales, Neuza Josina; Castaneda, Daniel Francisco Neyra; Barreto, Íkaro Daniel de Carvalho; Paoliello, Marina; Campanha, Silvia Márcia Andrade Abstract: Purpose: To describe how public prosecutors self-assess their communication approaches and how listeners react to them; to analyze how this relates to gender, age, and work experience. Methods: Descriptive, transversal study. A questionnaire was developed and sent to 126 public prosecutors for completion. Thirty-three completed questionnaires were sent back. The independent variables were gender, age, and number of years of professional experience. The dependent variables were communication self-assessment throughout the years of work, communication parameters used, and listeners’ reactions. A descriptive analyzis and Fisher’s Exact Test was carried out. Results: the sample contained both male and female participants with a median age of 43 years and an average of 20 years of professional experience. Most of the respondents claimed they had experienced demotivation, insecurity, tension, and difficulty when trying to convince listeners. More women than men reported they felt that their communication had worsened throughout their careers. All the women reported they experienced insecurity when speaking in public. One third of the public prosecutors stated they suffered from disorders on their voice. Those respondents aged over 43, experienced greater proportion on voice change than younger ones. In contrast to their younger colleagues, the majority of public prosecutors with more than 20 years of professional experience revealed that they felt insecure when speaking in public. Conclusion: the public prosecutors identified their strong and weak communication parameters. Gender, age, and work experience affect communicative performance. 2016-01-01T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/24128 Title: Intervenção nutricional educativa como ferramenta eficaz para mudança de hábitos alimentares e peso corporal entre praticantes de atividade física Authors: Teixeira, Pryscila Dryelle Sousa; Reis, Bruna Zavarize; Vieira, Diva Aliete dos Santos; Costa, Dayanne da; Costa, Jamille Oliveira; Raposo, Oscar Felipe Falcão; Wartha, Elma Regina Silva de Andrade; Mendes Netto, Raquel Simões Abstract: The scope of this study was to evaluate the effectiveness of two methods of educational nutritional intervention together with women who practice regular physical activities by fostering the adoption of healthy eating habits. The study population consisted of 52 women aged between 19 and 59 who frequented the Academia da Cidade Program in Aracaju in the State of Sergipe. The study was a randomized comparison of two intervention groups and was of the pre-test/post-test variety. The educational activities were based on two protocols – one less intensive (P1 Group) and one more intensive (P2 Group) – over a period of two months. The variables analyzed were nutritional knowledge, anthropometric measurements and changes in eating habits. The changes identified were improvement in eating habits and reduction in weight and Body Mass Index for the P2 group. The modifications identified referred mainly to increased consumption of fruit and vegetables, reduction of fat in cooking, reduction in the volume of food eaten per meal and increased meal frequency. In relation to nutritional knowledge, only 2 of the 12 questions showed significant changes. The most intensive method proved effective in changing dietary habits leading to weight loss. 2013-02-01T00:00:00Z