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Efeito da nutrição materna sobre o desenvolvimento da progênie de codornas de postura

Fri, 25 Apr 2025 00:00:00 GMT

Título: Efeito da nutrição materna sobre o desenvolvimento da progênie de codornas de postura Autor(es): Silva, Lucas Soares da Abstract: ABSTRACT: Supplementation of a blend with phytogenic additives in the diet of laying quail breeders can improve reproductive performance and egg quality, in addition to positively influencing the health and development of the progeny. Therefore, our objective was to evaluate the effect of adding a blend of essential oils, organic acids, curcumin, tannins, vitamin E and microencapsulated zinc to the maternal diet on the productive and reproductive parameters of Japanese quail breeders, as well as the effects of maternal nutrition on embryonic development and progeny at 15 days of age. At 84 days of age, 100 females with an average weight of 153.30 g and a laying rate of approximately 85% were distributed in a completely randomized design with two dietary treatments, reference diet (n = 50 birds) and reference diet with blend (n = 50 birds). The inclusion of blend in the maternal diet increased egg mass (p<0.05). There was also a significant difference (p<0.05) for the total hatching rate and fertile eggs, with the best results observed in the breeders that consumed the diet with the blend. No significant effect of the diet was observed on the laying rate, average egg weight, egg moisture loss and the number of preovulatory follicles (p>0.05). The embryos from the breeders that received the diet with blend were heavier at 15 days of incubation (p<0.05). There was no effect (p>0.05) of treatments on yolk sac weight and embryo length. Maternal diet did not influence birth weight or 15-day-old weight of the offspring (p>0.05). The length of the intestine at 15 days was greater in the quails of the breeders that received a blend diet (p<0.05). The addition of the blend composed of essential oils of cinnamon, eugenol, thymol and oregano, organic acids (citric acid, fumaric acid, sorbic acid and malic acid), curcumin, tannins, vitamin E and microencapsulated zinc, optimized the performance of the breeders, in addition to having shown a positive effect on the embryonic and intestinal development of 15-day-old quail chicks.

Transcriptoma de codornas japonesas é modulado por temperatura embrionária e desafio imune

Mon, 28 Jul 2025 00:00:00 GMT

Título: Transcriptoma de codornas japonesas é modulado por temperatura embrionária e desafio imune Autor(es): Conceição, Juliana dos Santos Abstract: Embryogenesis is a critical programming period, as environmental factors can modulate the physiology of organisms. In birds, incubation temperature has been widely studied as an inducer of genetic responses that influence embryonic development and response to post- hatch challenges. In this context, the present study aimed to investigate the effects of embryonic thermal manipulation on the immune response of Japanese quails (Coturnix coturnix japonica) challenged with lipopolysaccharide (LPS) after hatching, using transcriptome analysis (RNA- seq). For this purpose, an experiment was carried out in a 2x2 factorial design, with two incubation temperatures: control temperature (CTL - 37.5°C) and high temperature (AT - 39°C) and two inoculation statuses: quails inoculated with LPS (LPS) and quails not inoculated with 49 LPS (SLPS). Eggs remained at the respective treatment temperatures throughout the incubation period, and 25 days after hatching, chicks were challenged with LPS or saline (placebo). After inoculation, jejunal tissue was collected for gene expression analysis via RNA-seq. Incubation temperature significantly reduced (p<0.05) hatching rates but did not affect (p>0.05) the morphological performance of surviving embryos. LPS induced significant changes (p<0.05) in the spleen, indicating immune system activation. Transcriptomic analysis revealed differentially expressed genes (adjusted p<0.05) associated with metabolic processes, immune response, and oxidative stress, with distinct patterns between the contrasts evaluated (CTL+LPS 57 vs. CTL+SLPS and AT+LPS vs. AT+SLPS). Under control conditions, LPS induced the differential expression of genes, notably IL1R2, IL18BP, and S100A12, related to inflammatory modulation, mucosal barrier integrity, and energy metabolism. In birds incubated at high temperature, LPS triggered broad changes, activating pathways associated with inflammation, lipid homeostasis, protein control, energy metabolism (glycolysis, pentose and Krebs cycles), and vesicular trafficking, while suppressing genes crucial for adaptive immunity, angiogenesis, cellular differentiation, and epigenetic regulation. A conserved set of inflammatory genes (MMP7, PI3, SLC6A14) was upregulated (adjusted p<0.05) under both thermal conditions, suggesting activation of central defense mechanisms. We conclude that high temperature during incubation compromises hatching, but not the phenotype of the offspring; However, it profoundly modulates the molecular response to LPS, altering inflammatory, metabolic, and regenerative pathways, which may impact intestinal adaptation to future stresses.

Qualidade do leite, saúde da glândula mamária, padrões fisiológicos e comportamentais de vacas lactantes, em sistema compost barn no semiárido

Fri, 31 Jan 2025 00:00:00 GMT

Título: Qualidade do leite, saúde da glândula mamária, padrões fisiológicos e comportamentais de vacas lactantes, em sistema compost barn no semiárido Autor(es): Cruz, Suelange Oliveira Abstract: The objective of this study was to evaluate milk production and quality, mammary gland health status, thermal environment, bedding conditions, locomotion, physiological parameters, behavior and temperament of lactating dairy cows managed in a compost barn confinement system during the dry and rainy seasons in a semi-arid region. The study was carried out on a commercial farm in the municipality of Nossa Senhora da Glória, in the Alto Sertão region of Sergipe, using 30 Girolando cows. The variables analyzed included the thermal characterization of the environment, milk production and composition, somatic cell count (SCC), standard plate count (SPC), milk acidity, occurrence of mastitis, hock and udder hygiene, teat hyperkeratosis, as well as physiological, behavioral, lameness, bedding quality and thermal comfort indices. The results indicated that the time of year significantly influenced milk production and some parameters of milk quality, litter quality, and physiological and behavioral parameters. However, no significant differences were observed in the somatic cell count, standard plate count, animal hygiene conditions, occurrence of mastitis, teat hyperkeratosis, and lameness. Thus, it is concluded that the time of year influences physiological and behavioral aspects as well as some parameters of milk quality of lactating cows in a compost barn system.

Caracterização da carne, pele e expressão gênica do colágeno do tipo I em duas variedades de Tilápias do Nilo (Oreochromis Niloticus)

Tue, 28 Feb 2023 00:00:00 GMT

Título: Caracterização da carne, pele e expressão gênica do colágeno do tipo I em duas variedades de Tilápias do Nilo (Oreochromis Niloticus) Autor(es): Campos, Vinícius José