https://ri.ufs.br/jspui/handle/riufs/18886 Fri, 24 Apr 2026 12:30:09 GMT 2026-04-24T12:30:09Z http://ri.ufs.br:80/retrieve/221bc4df-6c2a-4aaa-8e26-7c6fb08481e5/PROBIO-logo.jpeg https://ri.ufs.br/jspui/handle/riufs/18886 https://ri.ufs.br/jspui/handle/riufs/23635 Título: Modelagem molecular do receptor canabinoide tipo-1 do papagaio-verdadeiro (Amazona aestiva): análise dos modos de acoplamento de fitocanabinoides Autor(es): Santos Júnior, Elpídio Vicente dos Abstract: The cannabinoid type-1 receptor (CB1) has received increasing attention from the scientific community due to its central role in maintaining homeostasis, particularly for its pharmacological functions in the nervous and immune systems. Present in all vertebrates, CB1 is also attracting interest due to the therapeutic potential of secondary metabolites from Cannabis sativa (Linnaeus, 1753), whose ligands exhibit high affinity for the canonical binding site, stimulating the development of drugs aimed at modulating the endocannabinoid system, including potential applications in wild bird medicine. In this study, a three-dimensional model of the CB1 receptor from Amazona aestiva (Linnaeus, 1758), a South American parrot species whose experimental structure has not yet been elucidated, was constructed. Thus, the target receptor was obtained by homology, refined in membranes using 500-ns molecular dynamics simulations with GROMACS, and used in the virtual screening of several C. sativa secondary metabolites compiled from the literature (567 compounds from de plant, plus the reference agonist). Molecular docking was performed in the receptor active site using the GOLD genetic algorithm, using the full agonist CP55,940 as a control. The results showed that cannabisins, the ten variants tested, exhibited a high tendency to interact with the CB1 receptor, surpassing even the reference ligand CP55,940. The model also predicted high affinity for cannabisol, a dimeric phytocannabinoid derived from Δ9-tetrahydrocannabinol, recognized for its potent CB1 agonist activity. Another relevant finding was cannabitwinol, a cannabidiol dimer associated with thermoregulatory receptors, whose high affinity suggests possible bivalent activity, expanding theoretical hypotheses about its mechanisms of action. Finally, this study highlights the applicability of in silico approaches in the screening and prioritization of promising drug candidates selective for CB1 from A. aestiva, providing support for experimental investigations into the pharmacological relevance of C. sativa metabolites in wild birds. Tue, 28 Jan 2025 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/23635 2025-01-28T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/23633 Título: Potencial antimicrobiano e antioxidante de óleos essenciais obtidos de diferentes genótipos de Lippia gracilis Schauer Autor(es): Oliveira, Weslei da Silva Abstract: Due to the increasing demand for new compounds with applicability in medicine, the food industry, pharmacology, and therapeutics, the use of essential oils has gained prominence in recent years. These compounds possess chemical properties that allow their application in different areas of biotechnology. Based on this, the aim of the present study was to evaluate the antimicrobial and antioxidant activities of essential oils from different genotypes of Lippia gracilis Schauer, as well as to apply a predictive model to analyze the microbial growth kinetics of pathogenic strains exposed to different pH conditions (5.0, 6.0, and 9.0), essential oil concentrations (1.32, 2.64, and 5.29 mg/mL), and pH + essential oil combinations. Antimicrobial activity was assessed using the disk diffusion method, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). Most strains showed high to extremely high sensitivity to the oils. Pseudomonas aeruginosa was the only bacterium not sensitive. MIC and MBC values ranged from 1.32 to 42.37 mg/mL. Antioxidant activity was determined using the ABTS method (656.2–1,185.0 μmol Trolox/L), FRAP method (1,296.8–2,656.2 μmol Trolox/L), and DPPH method (314.6–446.6 μmol Trolox/L). In the predictive experiments, the essential oil that showed the lowest MIC and MBC values against most bacteria was used. In treatments varying only oil concentrations, no growth was observed for most strains. Furthermore, the combination of pH + essential oil was effective in inhibiting or reducing bacterial growth rate and prolonging the lag phase duration. Overall, L. gracilis essential oils demonstrated excellent potential for application as natural antioxidants and antimicrobial agents. Thu, 09 Oct 2025 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/23633 2025-10-09T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/23013 Título: Atividade antimicrobiana dos chás comerciais e sua potencialização em combinação com antibióticos no tratamento de infecções gastrointestinais: um estudo in vitro Autor(es): Ramos, Lorena de Melo Menezes Abstract: The growing appreciation of natural foods with biological properties has placedteasamong the most consumed beverages worldwide, due to their phytochemical compositionandantioxidant, anti-inflammatory, and antimicrobial activities. This interest in natural therapiesisparticularly strong in Brazil, supported by its vast biodiversity and easy access toplant-basedresources. The present study aimed to investigate the antimicrobial activity of commercial teas,both individually and in combination with antibiotics, for the treatment of gastrointestinalinfections. Samples of lemon balm (Lippia alba), boldo (Peumus boldus), and espinheira-santa(Maytenus ilicifolia) were analyzed. The infusions were prepared by boiling and testedthroughagar diffusion, while the synergistic effect was assessed using the Checkerboard method. When tested alone, lemon balm and boldo showed activity against Salmonella enterica, withinhibition zones of 15 mm and 21 mm, corresponding to 61% and 47%sensitivity, respectively.Espinheira-santa demonstrated limited activity against Staphylococcus aureus (13 mm), equivalentto 28%, while none of the teas were effective against Escherichia coli (0%). In combinationwithantibiotics, promising results were observed: ampicillin increased Salmonella sensitivityby70%;cefepime demonstrated synergy of 65% against Salmonella and 40%against E. coli; andtetracycline showed the strongest effect, reaching up to 85% inhibition of Salmonellawhencombined with espinheira-santa, in addition to significant effects against E. coli andS. aureus.Gentamicin confirmed efficacy in 100% of the strains, while sulfazotrimshowednointeraction(0%). In conclusion, although teas present limited antimicrobial activity when usedalone, theircombination with antibiotics can substantially enhance effectiveness, representingapromisingtherapeutic strategy against gastrointestinal infections. Fri, 06 Jun 2025 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/23013 2025-06-06T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/22436 Título: Potencial antimicrobiano e antioxidante de extratos de Hancornia speciosa frente a patógenos causadores de infecções cutâneas Autor(es): Oliveira, Iracema Nascimento de Abstract: Infections caused by microorganisms pose a global challenge due to the increasing resistance to antimicrobials. The insufficient number of new therapeutic agents to address this issue characterizes what researchers refer to as the "post-antibiotic era." Pathogens such as Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa are frequently associated with hard-to-treat infections, especially skin infections, and may possess resistance mechanisms against antimicrobials considered first-choice therapies, resulting in high healthcare costs. In this context, researchers have been exploring plant extracts and essential oils as alternatives to conventional therapies. Hancornia speciosa, a tree native to Brazil, has been investigated for its therapeutic properties; however, few studies have assessed the antimicrobial effect of extracts from this plant prepared with different extraction solvents. This study aimed to develop extracts from the leaves and bark of H. speciosa and evaluate their antimicrobial activity against the microorganisms S. aureus, S. epidermidis, and P. aeruginosa, which are involved in dermatological infections. For this purpose, the extracts were produced using the Soxhlet serial exhaustive extraction method and cold maceration, followed by phenol and flavonoid quantification. Antioxidant activity was assessed using the DPPH radical assay. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were performed, as well as disk diffusion assays to evaluate synergism with commercial antibiotics. Phenol quantification revealed that the hydroethanolic bark extract had the highest total phenol content, followed by the ethyl acetate and hexane extracts. Regarding flavonoids, the chloroform extract from leaves (3196.66±5.74 EQ/mg) and the hexane extract from leaves (1352.50±9.01 EQ/mg), both extracted by Soxhlet, showed the highest concentrations. In the antioxidant activity test with DPPH, the hydroalcoholic bark extract obtained through cold maceration showed the best result, with an IC50 of 6.46 μg/mL and AAI of 15.47. The extracts demonstrated antimicrobial activity both individually and synergistically with commercial antimicrobials, with particular highlights for the hexane bark extract obtained by Soxhlet and the hydroethanolic bark extract obtained through cold maceration, which had MIC values of 1.25 mg/mL and 5 mg/mL, respectively. Overall, the bark extracts of H. speciosa proved to be superior to the leaf extracts in terms of antimicrobial and antioxidant activities. This study is the first to compare H. speciosa extracts prepared using Soxhlet with different solvents, as well as to compare leaf and bark extracts obtained through cold ethanol extraction. The results suggest that H. speciosa has potential as a source of bioactive compounds with significant activity against clinical pathogens and antioxidant properties, making it a promising therapeutic alternative for dermatological infections. Fri, 31 Jan 2025 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/22436 2025-01-31T00:00:00Z