https://ri.ufs.br/jspui/handle/riufs/2145 Thu, 09 Jul 2026 03:11:39 GMT 2026-07-09T03:11:39Z https://ri.ufs.br/jspui/handle/riufs/25370 Título: Prospecção, conservação, caracterização genética, química, morfoagronômica e sazonalidade em germoplasma de Croton blanchetianus Baill Autor(es): Costa, Rosemeire Santos Abstract: Croton blanchetianus Baill. is a medicinal and aromatic species native to the Caatinga biome, widely distributed in the Brazilian Northeast. It is notable for its high bioactive and apicultural potential, and it is listed by the Ministry of Environment as a priority species for the region due to its potential economic importance. Nevertheless, its natural occurrence area is frequently reduced, primarily due to anthropogenic interventions. In view of the imminent threats to the species’ genetic variability, the Federal University of Sergipe (UFS) studied natural populations and subsequently established a collection of C. blanchetianus in its Active Germplasm Bank (AGB) for Medicinal and Aromatic Plants at UFS. This initiative allows the development of strategies for conservation and sustainable use of the species. The present research aims to evaluate the genetic and chemical diversity of natural populations of C. blanchetianus from municipalities in the state of Sergipe, as well as to establish a collection, characterize the accessions morpho-agronomically, chemically, and seasonally, and assess the antioxidant activity of the accessions conserved in the AGB. In the first article, the genetic diversity and structure of six natural populations of C. blanchetianus from different municipalities in the state were evaluated, using ISSR markers. Low genetic variability among populations (6%) was observed, and it was not possible to differentiate genotypes according to their geographic origin. In the second article, the chemical diversity of the essential oil from 70 genotypes of C. blanchetianus, collected from six natural populations in Sergipe, was evaluated. The major compounds found were α-pinene (1.60–13.37%), limonene (0.30–17.54%), β-phellandrene (4.38–16.04%), 1,8-cineole (0.16–13.56%), (E)-caryophyllene (0.31–13.14%), germacrene D (0.33–10.60%), bicyclogermacrene (5.06–27.47%), and spathulenol (4.34–29.83%), highlighting the broad chemical diversity of the species. In the third article, the morphoagronomic and chemical diversity of 26 C. blanchetianus accessions was characterized and analyzed. High variability among accessions was observed for all evaluated morpho-agronomic descriptors, particularly plant height (87.00–236.50 cm), crown diameter (48.75–131.00 cm), leaf area (74.50–176.37 cm²), dry mass (83.17–333.00 g/plant), and essential oil content (0.60– 1.60%). Chemically, the accessions were mostly comprised of α-pinene (3.90–12.02%), limonene (0.88–10.72%), β-phellandrene (1.30–17.51%), 1,8-cineole (0.36–11.57%), (E)- caryophyllene (1.82–12.48%), germacrene D (0.38–10.12%), bicyclogermacrene (8.37– 29.13%), and spathulenol (5.57–28.04%). In the fourth article, the chemical profile of the essential oil from leaves and fruits of five accessions of C. blanchetianus was analyzed, revealing quantitative and qualitative differences between plant organs. Leaves had a predominance of sesquiterpenes (37.34–62.03%), whereas fruits had higher levels of monoterpenes (23.97–61.03%). The exclusive presence of myrtenyl acetate in fruit essential oil indicates its potential as a chemical marker. In the fifth article, the seasonal influence (three harvest periods) on agronomic variables, chemical composition, and antioxidant activity of essential oil from 26 accessions of C. blanchetianus was evaluated. Accessions CBL-403 and CBL-503 showed the highest oil content (1.93%), while CBL-503 (17.41 g/plant) and CBL507 (17.38 g/plant) exhibited the highest essential oil yields. Sesquiterpenes predominated in harvests 1 and 2 (59.02% and 62.01%, respectively), while monoterpenes increased in harvest 3 (40.01%). A high antioxidant potential was observed in accession CBL-301 (DPPH: 66.24%; ABTS: 92.51%). These results indicate that C. blanchetianus exhibits exploitable genetic, morpho-agronomic, and chemical variability, and that its essential oils show seasonal plasticity and antioxidant activity. Tue, 24 Feb 2026 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/25370 2026-02-24T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/25369 Título: Fenotipagem por Vant e aprendizado de máquina para predição da produtividade de milho em ambientes contrastantes de nitrogênio em região semiárida Autor(es): Santos, Barbara Nascimento Abstract: High-throughput phenotyping has contributed substantially to advances in agriculture and plant breeding programs. By using unmanned aerial vehicles (UAVs), its application has enabled the rapid acquisition of phenotypic data with high reliability and spatial resolution, promoting its adoption across diverse agricultural contexts. Despite these advances, several methodological limitations remain insufficiently explored, particularly when this approach is applied under conditions of environmental stress and high spectral homogeneity. This study aims to evaluate the accuracy of four predictive models, using regression and machine learning approaches to estimate the grain yield of maize half-sib progenies grown under contrasting nitrogen conditions, based on multispectral images obtained by UAV. The experiments were conducted during the 2024 growing season in two experimental areas located in the municipalities of Nossa Senhora da Glória and Graccho Cardoso, Sergipe, Brazil. A randomized block design with split plots and three replications was adopted, in which nitrogen levels (high and low) were assigned to the main plots and the half-sib progenies to the subplots. Multispectral drone images were acquired during three flights at heights of 60 and 80m throughout the crop cycle, corresponding to different phenological stages. Fourteen multispectral vegetation indices associated with canopy structure, chlorophyll content, and plant nutritional status were calculated. Four predictive approaches were evaluated: a classical linear model, a penalized regression model (LASSO), and two decision tree-based algorithms, Conditional Forest (Cforest) and Gradient Boosting Machine (GBM). Model performance was evaluated using the root mean square error (RMSE) and the mean absolute error (MAE), in addition to diagnostic analyses of the linear model assumptions and assessments of variable importance and SHAP values for the GBM and Cforest models. In both experimental areas and nitrogen conditions, the Cforest and GBM models consistently showed higher predictive accuracy than the linear approaches. Overall, images acquired at 60m resulted in superior model performance, highlighting the importance of higher spatial resolution for increased sensitivity to physiological variability within the canopy. Analyses of variable importance and SHAP values indicated that predictive contributions were concentrated in a limited subset of vegetation indices, particularly those sensitive to canopy structure and chlorophyll content. Furthermore, distinct predictive responses were observed among genotypes, depending on the modeling approach and environmental conditions. These results suggest that the acquisition and processing of spectral data can be optimized by prioritizing specific vegetation indices and strategically selected phenological stages. For predicting maize grain yield, the combined use of the Cforest and GBM models with vegetation indices such as TCARI, MCARI, and MSAVI2 is recommended. To increase the robustness and generalizability of these findings, future studies should evaluate the performance of such indices across a broader range of nitrogen levels, genotypes, and edaphoclimatic conditions. Mon, 26 Jan 2026 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/25369 2026-01-26T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/25368 Título: Efeito citotóxico do derivado indólico NM-01-28-22 em linhagem de carcinoma de pulmão Autor(es): Melo, Marcos Vinícius Barbosa de Abstract: Lung cancer is a neoplasm with a high mortality and incidence rate. The main treatments for lung cancer are chemotherapy, radiotherapy, and surgery, depending on the stage of the cancer in the patient. Chemotherapy is the most widely used therapeutic practice, but it has limitations due to adverse effects caused by its low systemic selectivity for normal cells compared to tumor cells. Many studies have demonstrated the importance of indoles and their derivatives in cancer research. Thus, indole and its derivatives allow for greater selectivity for tumor cells. Our study aims to evaluate the indole derivative NM-01-28-22 in cell inhibition in tumor cell lines and its cytotoxic effect on the lung carcinoma cell line. Initially, the compound was tested against cell lines to determine the most susceptible to the derivative. For this purpose, the Sulforhodamine B assay was used to evaluate the degree of inhibition of the NM-01-28-22 derivative in the three cell lines A549 (lung cancer), PC-3 (prostate cancer), and MCF-7 (breast cancer). For cytotoxicity assays in the A549 cell line, cell migration, clone formation, morphological alterations by DAPI and Phalloidin-Fitc staining, mitochondrial membrane potential by rhodamine 123 dye, and ROS production by the DCFH-DA probe were performed. Toxicity in human erythrocytes was verified by the hemolysis assay. In the analysis of the degree of inhibition, the values of the 50% inhibitory concentration (IC50) of the compound were obtained in the A549 (6.5 µM), MCF-7 (17.5 µM), and PC-3 (16.2 µM) cell lines. Given that the indolic derivative showed a low IC50 in A549 cells and considering its relevance in epidemiology, this cell line was used in subsequent cytotoxicity assays. The derivative inhibited cell migration and clone formation in A549 cells at all three concentrations. The derivative also induced loss of mitochondrial membrane potential and ROS production, suggesting cell death by apoptosis. The morphological changes evidenced by treatment with the derivative also show cell death in the A549 cell line. The derivative was tested at high concentrations in human erythrocytes and no toxic effect was observed. These results show that the derivative inhibited cell growth in three distinct tumor cell lines. It also showed a high cytotoxic effect in the A549 cell line without showing a toxic effect in human erythrocytes. Fri, 13 Feb 2026 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/25368 2026-02-13T00:00:00Z https://ri.ufs.br/jspui/handle/riufs/25367 Título: A efetividade do acesso à justiça e a ampliação das atividades notariais: uma análise à luz das garantias fundamentais processuais Autor(es): Santos, Ewerton Diego Justiniano Abstract: This dissertation analyzes the expansion of the duties of notarial services in the context of dejudicialization and multi-door justice, examining the extent to which extrajudicial procedures observe and ensure the effectiveness of constitutional procedural guarantees without undermining the essential core of the constitutionally outlined fair trial. Based on the observation of the structural limitations of state jurisdiction and the need to streamline the justice system, the study investigates the role of notarial services as qualified instruments of legal protection, inserted in a hybrid and complementary institutional model. The research adopts a qualitative, legaltheoretical approach based on a literature review, legal analysis, and examination of normative and regulatory acts, especially those issued by the National Council of Justice, as well as the 2015 Code of Civil Procedure. The results indicate that the expansion of notarial powers does not imply a departure from fundamental procedural guarantees, provided that it is conditioned on compliance with institutional and functional limits that guide notarial practice, ensuring procedural rationality, control, protection of trust, and legal certainty. It is concluded that the notarial route, when structured according to these parameters, can legitimately contribute to effective access to justice and the strengthening of the constitutional rule of law. Tue, 10 Feb 2026 00:00:00 GMT https://ri.ufs.br/jspui/handle/riufs/25367 2026-02-10T00:00:00Z