Desenvolvimento de metodologia para quantificação de fenóis presentes em fração aquosa de pirólise por GC/MS e UHPSFC

Abstract

The liquid product of the thermal decomposition of the biomass by the pyrolysis process is formed by the bio-oil fraction and the aqueous fraction. The aqueous phase can reach 30% w / w of the products formed during the process. Knowledge of your composition is of paramount importance to define what will be its destination route, whether in the reuse of compounds for industrial application or treatment of effluent for disposal. Analytical techniques such as Gas Chromatography with Mass Spectrometry (GC / MS) and Chromatography with ultra-high efficiency supercritical fluid (UHPSFC) were used to evaluate the composition of phenolic compounds present in the aqueous fractions of the residues of beans, pinecones, acerola, grape, pine nuts and guava. The analyzes in the GC / MS were performed in a qualitative way and then the method was optimized for the quantitative, an extraction had to be performed previously. For the UHPSFC analysis, tests were performed to optimize the analysis conditions, and finally, the phenols were quantified. Both methods presented a similar profile in relation to the total concentration of phenols in the aqueous fractions, the increase in concentration varied depending on the type of biomass used in pyrolysis and was follow order: bean pod <pine cone seed <acerola seed < guava seed <pine seed <grape seed. The analysis method proposed by UHPSFC proved to be suitable for phenols in pyrolysis water, in addition to the samples being able to be analyzed directly, was much shorter (21 min) when compared to the gas chromatography method (50 min).