Please use this identifier to cite or link to this item: https://ri.ufs.br/jspui/handle/riufs/23390
Document Type: Artigo
Title: Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Authors: Leite, Nadjma Souza
Lima, Aloísio André Bomfim de
Santana, José Carlos Curvelo
Lopes, Francisco Luiz Gumes
Lédo, Ana da Silva
Tambourgi, Elias Basile
Souza, Roberto Rodrigues de
Issue Date: Oct-2012
Resumo : This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola’s.
Keywords: Enzymatic activity
Bromelain
Pérola pineapple
Imperial pineapple
Production in vitro
ISSN: 1516-8913
Is part of: Brazilian Archives of Biology and Technology
Language: eng
Publisher / Institution : Instituto de Tecnologia do Paraná - Tecpar
Citation: LEITE, N. S. et al. Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation. Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 5, p. 647-652, set./out. 2012. Disponível em: https://www.scielo.br/j/babt/a/7PmvQZc6CdjX8vJ9vfksHfg/?lang=en. Acesso em: 8 out. 2025.
License: Creative Commons Atribuição-NãoComercial 4.0 Internacional (CC BY-NC 4.0)
URI: https://ri.ufs.br/jspui/handle/riufs/23390
Appears in Collections:DEQ - Artigos de periódicos

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