Avaliação antimicrobiana da aloe vera associada ao hidróxido de cálcio em enterococcus faecalis

Abstract

The success of endodontic treatment depends on an efficient chemical- mechanical preparation and proper filling of the root canal system. Enterococcus faecalis is one of the bacteria most commonly found in persistent endodontic infections. Of medicines are required biological and mechanical properties, highlighting its bactericidal activity. Objective: To evaluate the antimicrobial activity of Aloe vera isolated and in combination with calcium hydroxide in Enterococcus faecalis by the microdilution test. Materials and methods: The leaves of the Aloe vera passed through the lyophilization process. Experimental groups were divided into 05, and the controller (CTR) positive and negative distilled water + Aloe vera (AA) Distilled + calcium hydroxide water (HA) and, finally, calcium hydroxide + distilled water + Aloe vera (HAA). Four clinical bacterial strains of Enterococcus faecalis were tested, plus a standard line (ATCC), which went through the process of revitalization. It was performed microdilution test, where the results were observed in 12h, 24h, 48h and 72 hours. Results: the HA group showed bactericidal effect on a species with MIC 160μg/mL, bacteriostatic effect in two with 20-40μg/ml MIC, and two strains, including ATCC, they showed no effect in 12 hours. The AA group pointed bacteriostatic effect in 12 hours with MIC from 20-160μg/mL across all tested strains. In the HAA group only two strains reached MIC 320μg/mL noting bactericidal effect in 12 hours. The other species of this group did not show any effect in this experimental time. Conclusion: It was found in this study that the combination of Aloe vera and calcium hydroxide presented is bactericidal against strains LMA LMA 2 and 3 of Enterococcus faecalis, exhibiting MIC equal to or greater than 320μg/mL in 12 hours. However, there was bacteriostatic activity analysis of Aloe vera group which proved most successful mainly on the concentration of 160μg/ml in strains LMA 3 and LMA 7B in 12 hours.