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https://ri.ufs.br/jspui/handle/riufs/23868| Document Type: | Artigo |
| Title: | Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates |
| Authors: | Oliveira, Flávia Márcia Ruiz-de-Souza, Valéria Pereira, Maria Aparecida Campana Gontijo, Cristiano Machado |
| Issue Date: | Dec-2013 |
| Resumo : | One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100µl of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro stimulation with palmitoyl-Ova; additionally, both Ova and palmitoyl-Ova induced the basal expression of CD11b. These findings could be related with the strong T-cell proliferative response induced by palmitoyl-Ova. |
| Keywords: | Ovalbumin Palmitic acid Cellular immunity CD86 CD80 CD11b |
| ISSN: | 1678-4324 |
| Is part of: | Brazilian Archives of Biology and Technology |
| Language: | eng |
| Publisher / Institution : | Instituto de Tecnologia do Paraná - Tecpar |
| Citation: | OLIVEIRA, F. M. et al. Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, p. 962-970, nov./dez. 2013. Disponível em: https://www.scielo.br/j/babt/a/CbVKn5xKtwfXDsjVm5zrP3r/?lang=en. Acesso em: 17 nov. 2025. |
| License: | Creative Commons Atribuição-NãoComercial 4.0 Internacional (CC BY-NC 4.0) |
| URI: | https://ri.ufs.br/jspui/handle/riufs/23868 |
| Appears in Collections: | DESL - Artigos de periódicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| CostimulatoryMoleculesAntigenAtivatedPeritonealMacrophages.pdf | 135,45 kB | Adobe PDF |  View/Open |
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