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Document Type:	Artigo
Title:	Soluble CD40 ligand in sera of subjects exposed to Leishmania infantum infection reduces the parasite load in macrophages
Authors:	Oliveira, Fabrícia Alvisi de Barreto, Aline Silva Bomfim, Lays Gisele Santos Leite, Talita Rebeca de Souza Santos, Priscila Lima dos Almeida, Roque Pacheco de Silva, Ângela Maria da Duthie, Malcolm Scott Reed, Steven G. Moura, Tatiana Rodrigues de Jesus, Amélia Ribeiro de
Issue Date:	2015
Resumo :	Background: While CD40L is typically a membrane glycoprotein expressed on activated T cells and platelets that binds and activates CD40 on the surface on antigen presenting cells, a soluble derivative (sCD40L) that appears to retain its biological activity after cleavage from cell membrane also exists. We recently reported that sCD40L is associated with clinical resolution of visceral leishmaniasis and protection against the disease. In the present study we investigated if this sCD40L is functional and exerts anti-parasitic effect in L. infantuminfected macrophages. Methodology/Principal Findings: Macrophages from normal human donors were infected with L. infantum promastigotes and incubated with either sera from subjects exposed to L. infantum infection, monoclonal antibodies against human CD40L, or an isotype control antibody. We then evaluated infection by counting the number of infected cells and the number of parasites in each cell. We also measured a variety of immune modulatory cytokines in these macrophage culture supernatants by Luminex assay. The addition of sCD40L, either recombinant or from infected individuals’ serum, decreased both the number of infected macrophages and number of intracellular parasites. Moreover, this treatment increased the production of IL-12, IL-23, IL27, IL-15, and IL1β such that negative correlations between the levels of these cytokines with both the infection ratio and number of intracellular parasites were observed. Conclusions/Significance: sCD40L from sera of subjects exposed to L. infantum is functional and improves both the control of parasite and production of inflamatory cytokines of infected macrophages. Although the mechanisms involved in parasite killing are still unclear and require further exploration, these findings indicate a protective role of sCD40L in visceral leishmaniasis.
Keywords:	Leishmania infantum CD40 Ligand Glycoprotein Macrophages
ISSN:	1932-6203
Is part of:	PloS One
Language:	eng
Publisher / Institution :	Public Library of Science
Citation:	OLIVEIRA, F. A. et al. Soluble CD40 ligand in sera of subjects exposed to Leishmania infantum infection reduces the parasite load in macrophages. PloS One, San Francisco, v. 10, n. 10, 2015. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141265. Acesso em: 11 mar. 2026.
License:	Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
Identifier:	https://doi.org/10.1371/journal.pone.0141265
URI:	https://ri.ufs.br/jspui/handle/riufs/24737
Appears in Collections:	DESL - Artigos de periódicos DME - Artigos de periódicos

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