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https://ri.ufs.br/jspui/handle/riufs/25986| Tipo de Documento: | Artigo |
| Título : | Anti-cryptococcal activity of ethanol crude extract and hexane fraction fromOcimum basilicum var. Maria bonita: mechanisms of action and synergism withamphotericin B and Ocimum basilicum essential oil |
| Autor : | Cardoso, Nathalia Nogueira Rodrigues Alviano, Celuta Sales Blank, Arie Fitzgerald Arrigoni-Blank, Maria de Fátima Romanos, Maria Teresa Villela Cunha, Marcel Menezes Lyra da Silva, Antonio Jorge Ribeiro Alviano, Daniela Sales |
| Fecha de publicación : | 2017 |
| Resumen: | Context: Ocimum basilicum L. (Lamiaceae) has been used in folk medicine to treat headaches, kidney dis-orders, and intestinal worms.Objective: This study evaluates the anti-cryptococcal activity of ethanol crude extract and hexane fractionobtained from O. basilicum var. Maria Bonita leaves.Materials and methods: The MIC values for Cryptococcus sp. were obtained according to Clinical andLaboratory Standards Institute in a range of 0.3–2500 lg/mL. The checkerboard assay evaluated the associ-ation of the substances tested (in a range of 0.099–2500 lg/mL) with amphotericin B and O. basilicumessential oil for 48 h. The ethanol extract, hexane fraction and associations in a range of 0.3–2500 lg/mLwere tested for pigmentation inhibition after 7 days of treatment. The inhibition of ergosterol synthesisand reduction of capsule size were evaluated after the treatment with ethanol extract (312 lg/mL), hexanefraction (78 lg/mL) and the combinations of essential oil þ ethanol extract (78 lg/mL þ 19.5 lg/mL,respectively) and essential oil þ hexane fraction (39.36 lg/mL þ 10 lg/mL, respectively) for 24 and 48 h,respectively.Results: The hexane fraction presented better results than the ethanol extract, with a low MIC (156 lg/mLagainst C. neoformans T444 and 312 lg/mL against C. neoformans H99 serotype A and C. gattii WM779serotype C). The combination of the ethanol extract and hexane fraction with amphotericin B and essen-tial oil enhanced their antifungal activity, reducing the concentration of each substance needed to kill100% of the inoculum. The substances tested were able to reduce the pigmentation, capsule size andergosterol synthesis, which suggest they have important mechanisms of action.Conclusions: These results provide further support for the use of ethanol extracts of O. basilicum as apotential source of antifungal agents. |
| Palabras clave : | Basil Antifungal activity Cryptococcus neoformans Drug synergism Ergostero |
| ISSN : | 1744-5116 |
| Es parte de: | Pharmaceutical Biology |
| Idioma : | eng |
| Institución / Editorial : | Taylor & Francis |
| Citación : | CARDOSO, N. N. R. et al. Anti-cryptococcal activity of ethanol crude extract and hexane fraction fromOcimum basilicum var. Maria bonita: mechanisms of action and synergism withamphotericin B and Ocimum basilicum essential oil. Pharmaceutical Biology, London, v. 55, n. 1, p. 1380–1388, 2017. DOI: https://doi.org/10.1080/13880209.2017.1302483. Disponível em: https://www.tandfonline.com/doi/full/10.1080/13880209.2017.1302483#abstract. Acesso em: 1 set. 2026. |
| License: | Creative Commons Atribuição 4.0 Internacional (CC BY 4.0) |
| Identificador: | http://dx.doi.org/10.1080/13880209.2017.1302483 |
| URI : | https://ri.ufs.br/jspui/handle/riufs/25986 |
| Aparece en las colecciones: | DEA - Artigos de periódicos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| AntiCryptococalActivityEthanolCrudeExtract.pdf | 1,6 MB | Adobe PDF | Visualizar/Abrir |
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