O exercício físico neuromuscular agudo promove respostas nos níveis circulantes do miR-146a em idosos com diabetes do tipo 2

Abstract

Diabetes mellitus type 2 (DM2) is characterized by defects in the action and secretion of insulin and in the regulation of hepatic glucose production. The physical exercise appears as one of the main ways of prevention and control of DM2. The literature suggests that circulating microRNAs (126, 146a and 155) have an inversely proportional relationship between glycemic rates and the pro-inflammatory state resulting from DM2. Thus, the aim of this study was to analyze the acute effect of two types of interventions (cardiovascular and neuromuscular) on the total concentrations of circulating of the miRs 126, 146a and 155 in elderly patients with DM2. The study included 23 individuals (68.23±5.31 years), of both genders, 13 patients with DM2 (controlled) and 10 non-diabetics, referred to, Group 1 and 2, respectively. These were subjected to two types of interventions: neuromuscular circuit and walk oriented, carried out with the intensity between 60% to 70% of heart rate reserve. The expression of miRNAs was performed using the method Real Time Quantitative PCR (qPCR) and was adopted the analysis of covariance (ANCOVA) whereas the measurements of baseline as co-variable. In all analyzes was used a significance level of 5%. While main results verify that the baseline (co-variable), is significantly related with the magnitude of the effect of miR-146a forward to intervention neuromuscular F (1, 23) = 166.31; p<0.001. There was also a significant effect on circulating levels of miR-146a in the face of the patient or may not be a diabetic with elevations substantially higher than the elevations shown by the non-diabetics, in front of the intervention 1, F (2, 23) = 54.33, p<0.001. It is concluded that neuromuscular intervention, when performed as a circuit, changes the total circulating levels of miR-146a and may reduce the inflammatory levels resulting from DM2.