Desenvolvimento de metodologia para determinação de pesticidas em cerveja brasileira

Abstract

Beer is a drink produced from the fermentation of barley and flavored by hops. In Brazil according to the legislation part of the barley can be replaced by cereals with high content of starch like corn and rice. In order to achieve high levels of production of these cereals and to avoid the loss of crops, pesticides are used in crops. This work aims to develop a methodology for the determination of residues of the pesticides acetamiprid, alachlor, ametryn, atrazine, azoxystrobin, carbofuran, carbosulfan, cypermethrin, deltamethrin, diphenoconazole, esfenvalerate, flutriafol, methionine-parathion and thiamethoxam in beer. In the QuEChERS procedure two adsorbents were tested in the clean-up step, PSA and Florisil, which presented recovery values of 73-101% and 65-103%, respectively. In order to validate the analytical method, the following parameters were tested: linearity and sensitivity, which presented linear response with correlation coefficients between 0.9024-0.9999 in the concentration range 0.05- 2.5 μg mL-1; Accuracy and accuracy tests were performed resulting in recovery values between 29-123% and coefficients of variation between 0.3-49.5% for the concentrations of 0.3, 0.1 and 0.05 μg mL -1; and limits of detection and quantification limits 0.003-0.08 μg mL-1 and 0.01-0.12 μg mL-1, respectively. After validation, the method was applied in real samples of beer and no residue of the pesticides studied was found.