Please use this identifier to cite or link to this item:
Document Type: Artigo
Title: Viability of canafistula (Cassia grandis L.f.) seeds by the tetrazolium test
Authors: Santos, Heloisa Oliveira dos
Silva-Mann, Renata
Ferreira, Robério Anastácio
Von Pinho, Edila Vilela de Resende
Caldeira, Carla Massimo
Pires, Raquel Maria de Oliveira
Silva, Valkiria Fabiana da
Issue Date: Mar-2016
Resumo : Among the speed tests that allows to determine the viability and vigor of seeds, the tetrazolium test is one of the most promising alternative. However, it is necessary to establish a specific methodology for each species studied. Thus, this present work was realized with the aim to adequate the methodology of tetrazolium test to evaluate the viability of canafistula(Cassia grandis L. F.). Were evaluated the following procedures to the removal of seeds coat: moistening in paper towel and immersion in water by 24 hours 30°C in an incubator type BOD. Then, the embryos were immersed in 0,075%, 0,5% and 1% of tetrazolium solution at 30°C in the dark for 4, 8 and 12 hours for the color development. Between the tested procedures to the removal of seeds coat, the immersion of seeds in water at 30°C for 24 hours allows better imbibition of seeds. Uniform coloration was obtained when seeds were immersed in tetrazolium solution at 0,5% for 8 hours at 30°C in the dark. The vigorous tissue was colored by brilliant pink, while the tissues with some deterioration or with damages presented the coloration of red-carmine intense. It was possible to establish three viable classes of seeds (class 1, 2 and 3) and three non-viable and dead (class 4, 5 and 6).
Keywords: Speed tests
Forest seeds
ISSN: 0975-833X
Is part of: International Journal of Current Research
Language: eng
Citation: SANTOS, H. O. dos. et al. Viability of canafistula (Cassia grandis L.f.) seeds by the tetrazolium test. International Journal of Current Research, v. 8, n. 3, p. 28031-28035, mar. 2016. Disponível em: Acesso em: 20 ago. 2019.
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Appears in Collections:DCF - Artigos de periódicos

Files in This Item:
File Description SizeFormat 
ViabilityCanafistulaSeeds.pdf347,48 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.