Modelo de neurotuberculose experimental com cepa H37RV

Abstract

Introduction: Neurotuberculosis stands out among the most severe forms of extrapulmonary extrapulmonary tuberculosis. It predominantly affects children and those co-infected with TB-AIDS, leading to death in 19.3% and deaths in 19.3% and 40%, respectively. Despite the major contributions of the postulates of Rich and McCordock (1933), the cellular and molecular aspects of the pathogenesis remain obscure. In order to contribute to the elucidation of these aspects, the current study aims to develop an experimental an experimental model of neurotuberculosis in black mice. Methods: Eighteen C57Bl/6 mice were divided into three groups of six. Two groups were infected with a virulent strain of Mycobacterium tuberculosis, one intracerebroventricularly and the other intratracheally. intratracheal route. The remaining group was used as a negative control. After four weeks, all groups were sacrificed, and the collected organs (lungs and brains) were were submitted to qualitative-morphological analysis on slides stained by hematoxylin and eosin and Ziehl Neelsen. Ziehl Neelsen. Results: We evidenced cerebral edema, perivascular infiltrate extravasation of blood cells, ependymal disorganization and increased microglia cells on histology. the histology of animals infected with the virulent strain, but no granulomatous formations were found. granulomatous formations were not found. As well as the morphological preservation of the control animals of the control group, which received saline solution via stereotaxis. Conclusion: We demonstrated the establishment of neurotuberculosis in its meningeal presentation, with the intratracheal route, which brings this murine model closer to the infectious process in humans. humans. Moreover, the use of C57BL/6 mice greatly expands the feasibility of future immunobiological feasibility of future immunobiological studies due to their known possibilities of genetic manipulation. genetic manipulation.