Análise simultânea da losartana, metformina e sinvastatina por cromatografia líquida

Abstract

Therapeutic Drug Monitoring (TDM) is used to adjust the drug dose to each patient, with the individualization, and in the assessment of the patient's adherence to the pharmacotherapy. Furthermore, TDM is one of the pharmaceutical services that can be used to find the health needs of the patient, family and community, as it is a tool that, when used correctly, can assist in clinical decision making. In this sense, the objective is the development of a liquid chromatography method that simultaneously analyzes losartan, metformin and simvastatin. In this perspective, a literature review was carried out on active search platforms in national and international databases, using as keywords: therapeutic drug monitoring, pharmacokinetics, cost-effectiveness, losartan, metformin and simvastatin. Based on the results obtained, the methodologies were analyzed and those that most resembled the present laboratory conditions were selected for the development of the method. Soon after, the samples were prepared and injected in the High Efficiency Liquid Chromatograph (HPLC) to obtain the peaks. The mobile phase consisted of ultrapure water (solution A) and acetonitrile (solution B) at a gradient of: 0 min – 10% B, 5 min – 20% B, 40 min – 100% B, 41 min – 20% B, 50 min. After the analysis, we obtained retention times of 2.39; 21.47 and 39.25 min for metformin, losartan and simvastatin respectively. Through the work, it is concluded that it is possible to perform the three drugs analyses in the same chromatographic run through the HPLC method, resulting in an optimization analysis time. Thus, it is expected that the method unprecedented in the literature, after validation, will be used for drug therapeutic monitoring the Medical Clinic of the University Hospital of Aracaju patients who use losartan, metformin and/or simvastatin, providing pharmacotherapeutic monitoring of patients to reduce the morbidity, mortality and improve the quality of life of the studied population.