Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual

Abstract

Introduction: Naringenin (NAR) is an abundant flavonoid in citrus fruits and represents a promising compound for therapeutic purposes. It has been widely used in the face of its antioxidant, anti-inflammatory and anti-tumor activity. Objective: To evaluate the possible effects of naringenin incorporated into the membrane of gelatin containing liposome (GEL/NAR) on wound healing in a model of open cutaneous wounds in rodents. Material and Methods: Wistar male rats weighing 250-300 g were used to perform the experimental protocols. These were anesthetized intraperitoneally (Ketamine-80 mg / kg and Xylazine-10 mg / kg) and with an 8 mm metallic punch the excisional cutaneous wound was made in the dorsal region of the animal. After the procedure, the animals were treated for 7 days, single dose, with gelatine membrane containing liposome (BR), gelatine membrane containing liposome and 1% NAR (GEL/NAR 1%) and 2.5% (GEL/NAR 2.5%) or untreated (LP). The evaluation consisted of the measurement of the wound areas and the histological aspect regarding the tissue morphological characteristics after hematoxylin-eosin (HE) staining. The experimental protocols were approved by the Animal Research Ethics Committee (CEPA/UFS - 71/2015). Results: GEL/NAR 1% (56,16075 ± 3,653587; p<0,001) and GEL/NAR 2,5% (58,21967 ± 1,651126; p<0,001) reduced the area of the wound, when compared to the control group (37,41523 ± 6,276465). Regarding the intensity of the inflammatory response, LP, BR and NAR 1% were classified as moderate and GEL/NAR 2.5% as mild. According to the inflammatory phenotype, subacute inflammation was observed in the LP and BR groups due to the balance between neutrophils and lymphocytes and chronic nonspecific inflammation in the 1% and 2.5% NAR groups due to the predominance of lymphocytes. GEL / NAR 1% (80,000 ± 15,3188; p<0,01) and GEL / NAR 2.5% (75,2857 ± 17,3754; p<0,05)presented significant values regarding epithelization compared to LP (58,6571 ± 6,3892) and BR (51,5714 ± 11,0432),in addition demonstrated a greater vascularization and deposition of fibroblasts. Conclusion: In this sense, GEL/NAR provided improvement in the tissue repair evidenced by reduction of the wound area and regulation of the inflammatory reaction through the reduction of neutrophils and lymphocytes. Financial Support: CNPq, COPES/POSGRAP/UFS.