Termonocicepção na prole de ratas hipotireoidianas: comprometimento de componentes neuroquímicos

Abstract

Objective: To investigate the repercussions of experimental gestational hypothyroidism (I) on the neurochemical brain system in the rat prosthesis at 60 days postnatal. Materials and methods: For the induction of I, 0.02% methimazole (MTZ) was used in drinking water from the 9th day of gestation until the day of delivery. At 60 days postnatal (TLD), the male offspring of I (PME) and hypothyroid (PMH) mothers underwent a supra-spinal nociception evaluation by means of exposure to the hot plate apparatus at 52 ± 0.2 ° C, and the measurement of the time interval between exposure and vigorous licking of the hind paw. The testes were basally achieved and after i.p. of alpha-methyl-p-tyrosine (AMPT), tyrosine hydroxylase (TH) inhibitor (at 0 and 3 h post injection). Quantification of the lateral ventral portion of the periaqueductal gray matter (PAGvl) immunolabelled by TH and the quantification of glutamate in the cerebrospinal fluid was performed. 3 – way or 2 – way ANOVA, Bonferroni and Student ̳s t test were investigated when. Results: The quantification of glutamate in the liquid reduced the content of this neurotransmitter in PMH (p <0.01). Inhibition of HT did not alter the thermociceptive behavioral response. Likewise, the number of TH-positive neurons in PAGv1 did not differ between groups. Conclusion: Absence of maternal HTs, exclusively in intrauterine life, under the neurochemical principle of thermo-nociceptive control and can provoke thermo-nociceptive disorders that are of unknown origin.