Alterações morfológicas e determinação do nível de absorção de chumbo em pulmões de ratas WISTAR adultas tratadas com acetato de chumbo

Abstract

Lead is among the most widespread toxic heavy metals in the environment and can pose serious risks to human and animal health. This study aimed to study the morphological alterations in the lungs of adult Wistar rats treated with lead acetate and to quantify this heavy metal. Twenty female Wistar rats were dived into three experimental groups treated with concentrations of 100, 200 and 300 mg / kg of lead acetate, while the control group was treat with distilled water. Following the Standards of the Research Ethics Committee on Animals, after eight weeks the rats were sacrifice. For the structural analysis, histological sections were submit to the microtome technique and stained by the Masson trichrome. For dosing, the lead concentration was measure in an atomic absorption spectrophotometer with deuterium background corrector. Macroscopically, changes in organ size, volume, color and consistency were inversely associated with the increase in the concentration of lead acetate, causing a disorganization in their respective forms when compared as a control group. Microscopically, it was observe that in the lung tissue, the impregnation of lead acetate from the lower exposure resulted in a bronchial, peribronchial and alveolar inflammatory response and a decrease in light from the alveoli to the destruction of the same ones observed in the largest experimental exposure. The presence of angiogenesis in lung tissues is proportional to the increase in lead acetate concentrations. The absorption rates of lead found in the lungs through the atomic absorption spectrophotometry technique justified the presence of morphological changes.