1. Repositório Institucional da Universidade Federal de Sergipe - RI/UFS
  2. PRODUÇÃO CIENTÍFICA
  3. DMO - Departamento de Morfologia
  4. DMO - Artigos de periódicos
Use este identificador para citar ou linkar para este item: https://ri.ufs.br/jspui/handle/riufs/23515
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorScher, Ricardo-
dc.contributor.authorGarcia, Juliana Bório Ferreira-
dc.contributor.authorPascoalino, Bruno-
dc.contributor.authorSchenkman, Sergio-
dc.contributor.authorCruz, Angela Kaysel-
dc.date.accessioned2025-10-15T19:44:23Z-
dc.date.available2025-10-15T19:44:23Z-
dc.date.issued2012-05-
dc.identifier.citationSCHER, R. et al. Characterization of anti-silencing factor 1 in Leishmania major. Memórias do Instituto Oswaldo Cruz, São Paulo, v. 107, n. 3, p. 377-386, maio 2012. Disponível em: https://www.scielo.br/j/mioc/a/98YKnVrzr4wgWBvJZN85bQm/?lang=en. Acesso em: 15 out. 2025.pt_BR
dc.identifier.issn1678-8060-
dc.identifier.urihttps://ri.ufs.br/jspui/handle/riufs/23515-
dc.languageengpt_BR
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúdept_BR
dc.relation.ispartofMemórias do Instituto Oswaldo Cruzpt_BR
dc.subjectLeishmania majoreng
dc.subjectAnti-silencing factor 1eng
dc.subjectHistone chaperoneeng
dc.titleCharacterization of anti-silencing factor 1 in Leishmania majorpt_BR
dc.typeArtigopt_BR
dc.identifier.licenseCreative Commons Atribuição-NãoComercial 4.0 Internacional (CC BY-NC 4.0)pt_BR
dc.description.resumoAnti-silencing factor 1 (ASF1) is a histone chaperone that contributes to the histone deposition during nucleosome assembly in newly replicated DNA. It is involved in chromatin disassembly, transcription activation and in the cellular response to DNA damage. In Leishmania major the ASF1 gene (LmASF1) is located in chromosome 20 and codes for a protein showing 67% of identity with the Trypanosoma brucei TbASF1a. Compared to orthologous proteins, LmASF1 conserves the main residues relevant for its various biological functions. To study ASF1 in Leishmania we generated a mutant overexpressing LmASF1 in L. major. We observed that the excess of LmASF1 impaired promastigotes growth rates and had no impact on cell cycle progress. Differently from yeast, ASF1 overproduction in Leishmania did not affect expression levels of genes located on telomeres, but led to an upregulation of proteins involved in chromatin remodelling and physiological stress, such as heat shock proteins, oxidoreductase activity and proteolysis. In addition, we observed that LmASF1 mutant is more susceptible to the DNA damaging agent, methyl methane sulphonate, than the control line. Therefore, our study suggests that ASF1 from Leishmania pertains to the chromatin remodelling machinery of the parasite and acts on its response to DNA damage.pt_BR
dc.description.localRio de Janeiropt_BR
Aparece nas coleções:DMO - Artigos de periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
CharacterizationAntiSilencingFactorLeishmaniaMajor.pdf2,53 MBAdobe PDFVisualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.