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Tipo de Documento:	Artigo
Título:	A new device for step-down inhibitory avoidance task - effects of low and high frequency in a novel device for passive inhibitory avoidance task that avoids bioimpedance variations
Autor(es):	Borba Filho, Gilvan Luiz Zenk, Kamila Cagliari Kalinine, Eduardo Baggio, Suelen Pettenuzzo, Letícia Zimmer, Eduardo Rigon Weis, Simone Nardin Calcagnotto, Maria Elisa Souza, Diogo Onofre de
Data do documento:	2015
Resumo:	Background: Step-down inhibitory avoidance task has been widely used to evaluate aversive memory, but crucial parameters inherent to traditional devices that may influence the behavior analysis (as stimulus frequency, animal’s bioimpedance) are frequently neglected. New Method: We developed a new device for step-down inhibitory avoidance task by modifying the shape and distribution of the stainless steel bars in the box floor where the stimuli are applied. The bars are 2mm wide, with rectangular shape, arranged in pairs at intervals of 1cm from the next pairs. Each pair makes an electrical dipole where the polarity inverts after each pulse. This device also presents a component that acquires and records the exact current received by the animal foot and precisely controls the frequency of stimulus applied during the entire experiment. Result: Different from conventional devices, this new apparatus increases the contact surface with bars and animal´s paws, allowing the electric current pass through the animal´s paws only, drastically reducing the influence of animal’s bioimpedance. The analysis of recorded data showed that the current received by the animal was practically the same as applied, independent of the animal´s body composition. Importantly, the aversive memory was observed at specific stimuli intensity and frequency (0.35 or 0.5 mA at 62 and 125Hz but not at 0.20 mA or 20 Hz). Moreover, with this device it was possible to observe the well-known step-down inhibitory avoidance task memory impairment induced by guanosine. Conclusion: This new device offers a substantial improvement for behavioral analysis in step-down inhibitory avoidance task and allows us to precisely compare data from different animals with distinct body composition.
Palavras-chave:	Step-down inhibitory avoidance task Behavioral analysis Bioimpedance Animals
ISSN:	1932-6203
Parte de :	PloS One
Idioma:	eng
Instituição/Editora:	Public Library of Science
Citação:	BORBA FILHO, G. L. et al. A new device for step-down inhibitory avoidance task - effects of low and high frequency in a novel device for passive inhibitory avoidance task that avoids bioimpedance variations. PLoS ONE, San Francisco, v. 10, n. 2, 2015. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116000. Acesso em: 26 mar. 2026.
Licença:	Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
Identificador:	https://doi.org/10.1371/journal.pone.0116000
URI:	https://ri.ufs.br/jspui/handle/riufs/25067
Aparece nas coleções:	PROCFIS - Artigos de periódicos

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