Potencial antioxidante da polpa liofilizada do fruto do noni na congelação do sêmen ovino

Abstract

Aimed to evaluate the antioxidant potential of freeze-dried pulp of the Noni (Morindacitrifolia) in addition to a diluent for semen freezing, the concentrations of 2,35mg / mL; 4,70mg / mL and 4,70mg / mL on the in vitro viability of sheep sperm. A total of five ejaculates from two breeding race Santa Inês aged 1 and 2 years, the technique of artificial vagina were collected. Semen was divided into equal volumes, diluted in the means corresponding to each treatment, stored in straws of 0.25 ml, followed by cooling to - 0.25 ° C / min to 5 ° C, a time of stabilization of an hour and freezing at - 20 º C / min up to - 140 º C, when they were submerged in liquid nitrogen at - 196 ° C. the samples were thawed (37 ° C / 30 seconds) and evaluated for feasibility, computerized sperm kinetics, plasma membrane integrity, status of training and acrosome reaction and applied the test of slow thermoresistance test for parameters like motility, vigor and integrity of the plasma membrane by hypoosmotic test. The addition of the freeze-dried noni fruit pulp concentration of 4.70 mg / mL reduced lipid peroxidation of the means (p <0.5). However, after dilution of the semen, followed by freeze / thawing media containing higher concentrations 2,35mg / mL harmful (p <0.5) the total motility, progressive motility, average path velocity, progressive velocity, curvilinear velocity, amplitude of lateral head displacement, beat cross cross and concentration 4,70mg / mL on the straightness of ram spermatozoa, without affecting the integrity of the plasma membrane and the status of capacitation and acrosome reaction. At the concentrations used, the use of freeze-dried Noni pulp is recommended as an antioxidant ingredient in diluent for freezing ram semen.