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Document Type: Artigo
Title: Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm
Authors: Santos, Clesivan Pereira dos
Pinheiro, José Baldin
Zucchi, Maria Imaculada
Bajay, Miklos Maximiliano
Campos, Jaqueline Bueno de
Arrigoni-Blank, Maria de Fátima
Pinto, Jéssika Andreza Oliveira
Blank, Arie Fitzgerald
Issue Date: 2016
Resumo : The conservation of plants in germplasm banks ensures the characterization and availability of these resources for future generations. The present study used DNA markers to obtain genetic information about germplasm collections of Lippia sidoides and L. gracilis, which are maintained in an Active Germplasm Bank (AGB). Genetic variability of samples in the AGB was assessed using 12 combinations of amplified fragment length polymorphism (AFLP) primers (EcoRI/MseI). Twenty simple sequence repeat primers designed for L. alba were tested to determine their transferability in L. sidoides and L. gracilis. The AFLP markers generated 789 markers. The assessed loci exhibited a moderate Shannon diversity index (I = 0.42) in both species, suggesting that the conserved accessions possess an intermediate level of genetic diversity. Twelve microsatellite loci amplified satisfactorily, and nine loci were polymorphic in each species. A total of 23, 22, and 36 alleles, with an average of 2.5, 2.4, and 3.27 alleles per locus were identified for L. sidoides and L. gracilis accessions in the AGB, and Lippia sp sampled plants, respectively. Analyses of genetic structure permitted the identification of three different groups using both sets of markers, of which two were representative of L. sidoides. The information generated in this study may help to create, expand, and maintain collections of these species and may assist in genetic-breeding programs.
Keywords: Lippia sidoides
Lippia gracilis
Medicinal plants
AFLP markers
Plantas medicinais
ISSN: 1676-5680
Is part of: Genetics and Molecular Research
Language: eng
Publisher / Institution : Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)
Citation: SANTOS, C. P. dos et al. Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, 2016. Disponível em: <>. Acesso em: 01 fev. 2018.
License: Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0)
Appears in Collections:DEA - Artigos de periódicos

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