1. Repositório Institucional da Universidade Federal de Sergipe - RI/UFS
  2. PRODUÇÃO CIENTÍFICA
  3. DZO - Departamento de Zootecnia (São Cristóvão)
  4. DZO - Artigos de periódicos
Use este identificador para citar ou linkar para este item: https://ri.ufs.br/jspui/handle/riufs/8493
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dc.contributor.authorNascimento, Carlos Souza do-
dc.contributor.authorBarbosa, Leandro Teixeira-
dc.contributor.authorBrito, Claudson-
dc.contributor.authorFernandes, Roberta Pereira Miranda-
dc.contributor.authorSilva-Mann, Renata-
dc.contributor.authorPinto, Ana Paula Gomes-
dc.contributor.authorOliveira, Haniel Cedraz de-
dc.contributor.authorDodson, Mike V.-
dc.contributor.authorGuimarães, Simone Eliza Facioni-
dc.contributor.authorDuarte, Marcio de Souza-
dc.date.accessioned2018-06-26T23:26:52Z-
dc.date.available2018-06-26T23:26:52Z-
dc.date.issued2015-05-
dc.identifier.citationNASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018.pt_BR
dc.identifier.issn1932-6203-
dc.identifier.urihttp://ri.ufs.br/jspui/handle/riufs/8493-
dc.description.sponsorshipConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqpt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpt_BR
dc.description.sponsorshipFundação de Apoio a Pesquisa e à Inovação Tecnológica do Estado de Sergipe - FAPITEC/SEpt_BR
dc.languageporpt_BR
dc.publisherPLOSpt_BR
dc.relation.ispartofPLOS Onept_BR
dc.subjectAvespor
dc.subjectGenespor
dc.subjectGallus galluspor
dc.subjectFrangospor
dc.subjectChickeneng
dc.titleIdentification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)pt_BR
dc.typeArtigopt_BR
dc.identifier.licenseCreative Commons Atribuição 4.0 Internacional (CC BY 4.0)pt_BR
dc.description.resumoThirteen reference genes were investigated to determine their stability to be used as a housekeeping in gene expression studies in skeletal muscle of chickens. Five different algorithms were used for ranking of reference genes and results suggested that individual rankings of the genes differed among them. The stability of the expression of reference genes were validated using samples obtained from the Pectoralis major muscle in chicken. Samples were obtained from chickens in different development periods post hatch and under different nutritional diets. For gene expression calculation the ΔΔCt approach was applied to compare relative expression of pairs of genes within each of 52 samples when normalized to mitochondrially encoded cytochrome c oxidase II (MT-CO2) target gene. Our findings showed that hydroxymethylbilane synthase (HMBS) and hypoxanthine phosphoribosyl transferase 1 (HPRT1) are the most stable reference genes while transferrin receptor (TFRC) and beta-2-microglobulin (B2M) ranked as the least stable genes in the Pectoralis major muscle of chickens. Moreover, our results revealed that HMBS and HPRT1 gene expression did not change due to dietary variations and thus it is recommended for accurate normalization of RT-qPCR data in chicken Pectoralis major muscle.pt_BR
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