Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1

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Campo DC	Valor	Idioma
dc.contributor.author	Cândido, Alexandre Luna	-
dc.contributor.author	Bontempo, Elizabete Dias	-
dc.contributor.author	Resende, Maurício	-
dc.date.accessioned	2014-02-03T19:22:58Z	-
dc.date.available	2014-02-03T19:22:58Z	-
dc.date.issued	2010-04	-
dc.identifier.citation	CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.	pt_BR
dc.identifier.issn	0074-0276	-
dc.identifier.uri	https://ri.ufs.br/handle/riufs/865	-
dc.description.abstract	Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.	pt_BR
dc.language.iso	en	pt_BR
dc.publisher	Fundação Oswaldo Cruz	pt_BR
dc.subject	Herpesvirus bovino	pt_BR
dc.subject	Co-solventes	pt_BR
dc.subject	DNA	pt_BR
dc.subject	Reação em cadeia da polimerase	pt_BR
dc.title	Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1	pt_BR
dc.type	Artigo	pt_BR
dc.identifier.license	Creative Commons Attribution License	pt_BR
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