Extração de compostos bioativos de zingiber officinale roscoe e avaliação da sua atividade frente a fatores de virulência de staphylococcus aureus

Abstract

INTRODUCTION: Zingiber officinale presents as main constituents the gingerois, substances responsible for most of its therapeutic activities, among them, antimicrobial. There are few studies reporting the antimicrobial activity of the Z. officinale species and none evaluating the activity against virulence factors of Staphylococcus aureus, bacterium causing various infections. One way to combat these infections is inhibiting their virulence factors, such as DNases, lipases and biofilm formation. OBJECTIVE: To extract and isolate substances from Zingiber officinale for evaluation of antimicrobial activity and of anti-virulence factors effect of methicillin resistant S. aureus. METHODS: The extracts obtained by maceration and turboextraction, in ethanol and hexane and the isolated substances by classical liquid chromatography and high performance liquid chromatography were tested to evaluate antimicrobial activity, anti-DNase, anti-lipase and biofilm formation. RESULTS: All extracts (T1: turboextraction, hexane; T2: turboextraction, ethanol; T3: maceration, hexane; T4: maceration, ethanol; 0.5 mg/mL) and substances (0.2 mg / mL), 6-, 8-, 10-gingerol and 6-shogaol showed activity against DNase. T1, T2, M1 and M2 inhibited the DNAse in 33.9%, 28.8%, 34.7% and 26.1%, respectively, whereas only the extracts T1 (17.49%) and T2 (18%) were active against lipase. Six-, 8- and 10-gingerol showed activity of 32.9%, 44.6% and 30.3% anti-DNase and showed inhibition of biofilm formation 39.8%, 65.9% and 52, 6%, respectively. In addition to inhibiting DNase, 6-shogaol was also shown to have a high antimicrobial potential, with a minimum inhibitory concentration (MIC) of 50 μg/mL. CONCLUSION: With these results it is concluded that it is of great relevance to find bioactive substances with antimicrobial potential and anti-virulence factors, to make them applicable in the treatment of infections caused by multiresistant bacteria.