Efeito do extrato de Anadenanthera colubrina na cicatrização de feridas cutâneas excisionais induzidas em camundongos

Abstract

Introduction: Wound healing is a complex skin repair process that can be impaired due to exacerbated inflammation, causing consequences physical, psychological and economic. In this sense, medicinal plants are promising alternatives in wound repair, mainly due to the modulation of inflammation. Anadenanthera colubrina, popularly known as angico, has been widely used in traditional medicine and has important medicinal properties anti-inflammatory, antioxidant, antimicrobial and healing. However, studies on skin repair remain scarce and scarring mechanisms are little explored. Objective: To evaluate the effect of the Ethanol Extract of A. colubrina (EEAc) on the healing of excision cutaneous wounds induced in mice. Methodology: Swiss female mice were submitted to dorsothoracic cutaneous excision with a 6 mm metallic punch and distributed in a control group, vehicle or EEAc 5%. The wound area was measured on days 0, 3, 5, 7, 10 and 14 and the tissues collected on days 1, 3, 7 and/or 14 to determine the activity of Myeloperoxidase (MPO), quantification of N-acetyl- β-D-glycosaminidase (NAG), measurement of TNF-α and IL-10 and analysis of leukocyte infiltrate, angiogenesis, re-epithelialization and collagen deposition in lesions. Results: The EEAc 5% induced a reduction (p <0.001) of the wound area on the 3rd, 5th and 7th day and increased collagen deposition (p <0.01) on the 14th day. In addition, the EEAc 5% group showed a reduction in MPO activity on the 3rd day, when compared to the control (p <0.01) or vehicle (p <0.001), and an increase in NAG on the 7th day (p <0.001). No differences were observed for TNF-α, but there was an increase in the amount of IL-10 on the 3rd day (p <0.01). Conclusion: The EEAc 5% was effective in healing excisional wounds in mice through the effects of reducing area and neutrophil infiltrate, increasing macrophage infiltrate, anti-inflammatory cytokine IL-10 and collagen deposition in lesions.