Use este identificador para citar ou linkar para este item: http://ri.ufs.br/jspui/handle/riufs/1589
Tipo de Documento: Artigo
Título: Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
Autor(es): Silva, Thais Freitas da
Vollú, Renata Estebanez
Jurelevicius, Diogo
Alviano, Daniela Sales
Alviano, Celuta Sales
Blank, Arie Fitzgerald
Seldin, Lucy
Data do documento: Fev-2013
Abstract: Background: Lippia sidoides Cham., also known as pepper-rosmarin, produces an essential oil in its leaves that is currently used by the pharmaceutical, perfumery and cosmetic industries for its antimicrobial and aromatic properties. Because of the antimicrobial compounds (mainly thymol and carvacrol) found in the essential oil, we believe that the endophytic microorganisms found in L. sidoides are selected to live in different parts of the plant. Results: In this study, the endophytic microbial communities from the stems and leaves of four L. sidoides genotypes were determined using cultivation-dependent and cultivation-independent approaches. In total, 145 endophytic bacterial strains were isolated and further grouped using either ERIC-PCR or BOX-PCR, resulting in 76 groups composed of different genera predominantly belonging to the Gammaproteobacteria. The endophytic microbial diversity was also analyzed by PCR-DGGE using 16S rRNA-based universal and group-specific primers for total bacteria, Alphaproteobacteria, Betaproteobacteria and Actinobacteria and 18S rRNA-based primers for fungi. PCR-DGGE profile analysis and principal component analysis showed that the total bacteria, Alphaproteobacteria, Betaproteobacteria and fungi were influenced not only by the location within the plant (leaf vs. stem) but also by the presence of the main components of the L. sidoides essential oil (thymol and/or carvacrol) in the leaves. However, the same could not be observed within the Actinobacteria. Conclusion: The data presented here are the first step to begin shedding light on the impact of the essential oil in the endophytic microorganisms in pepper-rosmarin.
Palavras-chave: Lippia sidoides
Essential oil
Stem
Leaf
Endophytic bacteria and fungi
Plant-microorganism interaction
Óleos essenciais
Folha
Bactérias
Fungos
ISSN: 1471-2180
Instituição/Editora: BioMed Central
Citação: SILVA, T. F. et al. Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?. BMC Microbiology, v. 13, n. 29, fev. 2013. Disponível em: <http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-29>. Acesso em: 14 jan. 2016.
Licença: Creative Commons Attribution License 4.0
URI: https://ri.ufs.br/handle/riufs/1589
Aparece nas coleções:DEA - Artigos publicados em periódicos

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