Coleção de fungos fitopatogênicos para pesquisa científica e estudos acadêmicos

Abstract

The conservation of fungal isolates has been important in the maintenance of microorganisms, which contributes to studies about control of diseases caused by plant pathogens. In order to solve the plant disease problem, studies and research are carried out in order to develop effective methods in the field and so avoid high losses. Therefore, some microorganisms are already being used, making it necessary to explore new genera of species for future research and database collection. In this sense, in order to obtain new species for research and academic studies, the work consists of isolating phytopathogenic fungi and creating a collection of fungal isolates of agricultural importance, in addition to creating an electronic spreadsheet for identifying and organizing the fungi isolates, regarding their morphology, host plant and attachment of microscopic images. To carry out the work, a survey was made according to the symptoms observed in the field, then the material was stored in a humid environment, for the formation of the reproductive structure. In the laboratory, solid culture medium was prepared using the PDA method, and the fungi were isolated using direct and indirect methods. After isolation, they were transferred to an incubator with temperature at 25°C and a 12- hour photoperiod. After isolation, the identification was performed using morphological methods, both by characteristics of the reproductive structures and the characterization of the colonies. Following the methodology proposed in the work, exsiccates were created with material containing fungal symptoms. In the isolations, carried out with samples of plants with symptoms of phytopathogenic fungi, results were obtained, and of the isolated fungi, only two were added to the silica gel preservation method, namely Asperisporium caricae and Clarohilum henningsii.