Análise da associação da fotobiomodulação a laser no ligamento periodontal de dentes reimplantados de ratos: estudo piloto

Abstract

Abstract: Objective: Analyze, by histological analysis, the effect of an association between two wavelengths 808 and 660 nm of laser photobiomodulation ( LPBM ) in blood vessel formation in periodontal ligament of reimplanted teeth in Winstar rats. Materials and Methods: Twenty rats composed the groups, they were divided randomly into four groups (n = 5) according to the treatment before and after tooth reimplantation , as follows: GP - exposed to the dry environment, without LPBM ; GPL - exposed to the dry environment, and LPBM ; GLI - conservation in whole milk without LPBM ; GLIL - conservation in whole milk and LPBM . Then the canals were prepared and filled with calcium hydroxide paste. The rats were euthanized 15 days after reimplantation . The right hemimaxila including the central incisor reimplanted was removed, fixed in 10 % formalin and processed for histological hematoxylin - eosin (HE). The slides were evaluated by comparative descriptive analysis, Original magnification of 400X using semi-quantitative criteria for the presence of blood vessels. Results: The GP and GLI groups had fewer blood vessels without statistically significant differences between them. While GPL and GLIL groups had higher proliferation of blood vessels, however no statistically significant difference between them was found. However, there was a highly significant difference between groups GP and GPL as well as GLI and GLIL. Conclusion: It was concluded that the combination of FBML and whole milk as the storage allows greater formation of blood vessels in the periodontal ligament of reimplanted teeth.