Extraction of DNA from honey and its amplification by PCR for botanical identification

Jain, Sona Arun; Jesus, Flávia Thalita de; Marchioro, Giulia Manso; Araújo, Edílson Divino de

Please use this identifier to cite or link to this item: https://ri.ufs.br/jspui/handle/riufs/23778

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DC Field	Value	Language
dc.contributor.author	Jain, Sona Arun	-
dc.contributor.author	Jesus, Flávia Thalita de	-
dc.contributor.author	Marchioro, Giulia Manso	-
dc.contributor.author	Araújo, Edílson Divino de	-
dc.date.accessioned	2025-11-06T20:55:52Z	-
dc.date.available	2025-11-06T20:55:52Z	-
dc.date.issued	2013-12	-
dc.identifier.citation	JAIN, S. A. et al. Extraction of DNA from honey and its amplification by PCR for botanical identification. Food Science and Technology, Campinas, v. 33, n. 4, p. 753-756, out./dez. 2013. Disponível em: https://www.scielo.br/j/cta/a/F4ML8MYjkzkw3HLP9hTtSBg/?lang=en. Acesso em: 6 nov. 2025.	pt_BR
dc.identifier.issn	0101-2061	-
dc.identifier.uri	https://ri.ufs.br/jspui/handle/riufs/23778	-
dc.language	eng	pt_BR
dc.publisher	Sociedade Brasileira de Ciência e Tecnologia de Alimentos	pt_BR
dc.relation.ispartof	Food Science and Technology	pt_BR
dc.subject	Botanical origin	eng
dc.subject	Honey pollen	eng
dc.subject	Molecular approach	eng
dc.title	Extraction of DNA from honey and its amplification by PCR for botanical identification	pt_BR
dc.type	Artigo	pt_BR
dc.identifier.license	Creative Commons Atribuição-NãoComercial 4.0 Internacional (CC BY-NC 4.0)	pt_BR
dc.description.resumo	The physiochemical and biological properties of honey are directly associated to its floral origin. Some current commonly used methods for identification of botanical origin of honey involve palynological analysis, chromatographic methods, or direct observation of the bee behavior. However, these methods can be less sensitive and time consuming. DNA-based methods have become popular due to their simplicity, quickness, and reliability. The main objective of this research is to introduce a protocol for the extraction of DNA from honey and demonstrate that the molecular analysis of the extracted DNA can be used for its botanical identification. The original CTAB-based protocol for the extraction of DNA from plants was modified and used in the DNA extraction from honey. DNA extraction was carried out from different honey samples with similar results in each replication. The extracted DNA was amplified by PCR using plant specific primers, confirming that the DNA extracted using the modified protocol is of plant origin and has good quality for analysis of PCR products and that it can be used for botanical identification of honey.	pt_BR
dc.description.local	Campinas	pt_BR
Appears in Collections:	DBI - Artigos de periódicos

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