Avaliação in vitro da atividade antifúngica do Schinus Terebinthif Olius Raddi contra espécies de Candida SPP

Abstract

The denture stomatitis (DE) is a pathology characterized by a moderate or intense inflammatory process, presenting an erythematous, diffuse or dotted aspect of the palatal mucosa under the prosthesis, in addition to edema and congestion. The etiology of the disease is multifactorial, however, the use of poorly sanitized prostheses, smoking, nutritional deficiency and poor oral hygiene may act as predisposing factors, although candida infection is considered the main etiological factor. The Candida albicans is the most commonly associated fungal species in patients with DE, and muco suported prostheses are considered to be potential facilitators of DE. The treatment consists on the combination of antifungal and hygiene of the prosthesis. However, an increase in yeast resistance to existing treatments is observed. Thus, it is urgent to evaluate new compounds with antifungal characteristics and new therapeutic schemes for the treatment of oral fungal infections. The aim of our study was to evaluate in vitro the antifungal activity of Schinus Terebinthifolius Raddi (STR) against species of Candida spp. Initially we evaluated the antifungal activity of the STR through the test disc diffusion and microdilution in broth, and, posteriorly, we evaluated the data through descriptive statistics and the Kruskal- Wallis test using GraphPad 5.0. The fusion disk method showed that all four species of Candida spp. were sensitive (halo >10 mm) at the STR at 80%, 90% and 100% concentrations respectively. Microdilution assays demonstrated that STR reduced the viability of C. albicans in 97.46% (p <0.0064), 97.37% (p <0.0064), 95.78% (p <0. 05), 94.78% (p <0.05) and 94.38% (p <0.05) in the concentrations of 10%, 8%, 6%, 4% and 2% respectively. For C. glabrata, STR decreases the viability with at concentrations of 10%, 8% and 6%, presenting a reduction of 92.43% (p <0.05), 91.91% (p <0, 05) and 91.87% (p <0.05) respectively. The species C. tropicalis and C. krusei presented a similar behavior with reductions in viability at concentrations of 10% (p <0.05), 8% (p <0.05) and 6% (p <0.05). Thus, we can conclude that STR has antifungal activity on different species of Candida spp, being a promising phytotherapeutic medicine for the treatment of DE.