1. Repositório Institucional da Universidade Federal de Sergipe - RI/UFS
  2. PRODUÇÃO CIENTÍFICA
  3. DESL - Departamento de Educação em Saúde
  4. DESL - Artigos de periódicos
Use este identificador para citar ou linkar para este item: https://ri.ufs.br/jspui/handle/riufs/25068
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorFontoura, Isabela Cardoso-
dc.contributor.authorTrombone, Ana Paula Favaro-
dc.contributor.authorAlmeida, Luciana Previato de-
dc.contributor.authorLorenzi, Julio Cesar Cetrulo-
dc.contributor.authorRossetti, Renata Ariza Marques-
dc.contributor.authorMalardo, Thiago-
dc.contributor.authorPadilha, Everton-
dc.contributor.authorSchluchting, William Roberto-
dc.contributor.authorSilva, Ricardo Luís Louzada da-
dc.contributor.authorGembre, Ana Flávia-
dc.contributor.authorFiuza, José Eduardo Cury-
dc.contributor.authorSilva, Celio Lopes-
dc.contributor.authorPanunto-Castelo, Ademilson-
dc.contributor.authorCoelho-Castelo, Arlete Aparecida Martins-
dc.date.accessioned2026-07-06T17:55:06Z-
dc.date.available2026-07-06T17:55:06Z-
dc.date.issued2015-
dc.identifier.citationFONTOURA, I. C. et al. B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 12, p. 1095–1100, 2015. Disponível em: https://www.scielo.br/j/bjmbr/a/ZQMMVKbsG4CnF98hMb4XtdP/?lang=en. Acesso em: 1 abr. 2026.pt_BR
dc.identifier.issn1414-431X-
dc.identifier.urihttps://ri.ufs.br/jspui/handle/riufs/25068-
dc.languageengpt_BR
dc.publisherAssociação Brasileira de Divulgação Científicapt_BR
dc.relation.ispartofBrazilian Journal of Medical and Biological Researchpt_BR
dc.subjectDNA-Hsp65 vaccineeng
dc.subjectMemory T cellseng
dc.subjectB cellseng
dc.titleB cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunizationpt_BR
dc.typeArtigopt_BR
dc.identifier.licenseCreative Commons Atribuição 4.0 Internacional (CC BY 4.0)pt_BR
dc.description.resumoIn DNA vaccines, the gene of interest is cloned into a bacterial plasmid that is engineered to induce protein production for long periods in eukaryotic cells. Previous research has shown that the intramuscular immunization of BALB/c mice with a naked plasmid DNA fragment encoding the Mycobacterium leprae 65-kDa heat-shock protein (pcDNA3-Hsp65) induces protection against M. tuberculosis challenge. A key stage in the protective immune response after immunization is the generation of memory T cells. Previously, we have shown that B cells capture plasmid DNA-Hsp65 and thereby modulate the formation of CD8+ memory T cells after M. tuberculosis challenge in mice. Therefore, clarifying how B cells act as part of the protective immune response after DNA immunization is important for the development of more-effective vaccines. The aim of this study was to investigate the mechanisms by which B cells modulate memory T cells after DNA-Hsp65 immunization. C57BL/6 and BKO mice were injected three times, at 15-day intervals, with 100 mg naked pcDNA-Hsp65 per mouse. Thirty days after immunization, the percentages of effector memory T (TEM) cells (CD4+ and CD8+/CD44high/CD62Llow) and memory CD8+ T cells (CD8+/CD44high/CD62Llow/CD127+) were measured with flow cytometry. Interferon g, interleukin 12 (IL-12), and IL-10 mRNAs were also quantified in whole spleen cells and purified B cells (CD43– ) with real-time qPCR. Our data suggest that a B-cell subpopulation expressing IL-10 downregulated proinflammatory cytokine expression in the spleen, increasing the survival of CD4+ TEM cells and CD8+ TEM/CD127+ cells.pt_BR
dc.description.localRibeirão Pretopt_BR
dc.identifier.doihttps://doi.org/10.1590/1414-431X20154409-
Aparece nas coleções:DESL - Artigos de periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
BCellsExpressingIL-10mRNAModulateMemory.pdf275,09 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.