Desenvolvimento de método analítico para determinação de resíduos de pesticidas em mel de abelhas Apis Mellifera

Abstract

Honey, main beekeping product, used as food, sweetneer and to terapic purpose, has the image of being natural, healthy and clean. However, today this product is produced in a poluted environmental by different sources of contamination such as pesticides that diffuse around the world and can reach the population by the comsumption of contaminated food, as honey. The matrix solid phase dispersion (MSPD) combined to the modern chromatographic techniques such as gas chromatography (GC) is an alternative to avoid the different inconvenient found in the classical methods extraction. The present study develops a procedure for extraction of pirimicarb, malathion, bifenthrin and procymidone residue pesticides in honey Apis mellifera bee by MSPD and GC analysis with mass spectrometry detector (MS). In order to obtain a more adequate method for extraction their pesticides, assays with Florisil, silica, alumina and C18 were carried out twice to the appropriate sorvent choic as such as amounts and kinds of solvents by elution such as dichloromethane, acetonitrile, ethyl acetate and n-hexane. The efficiency of the method was determined in terms of mean percentual recoveries of the pesticides by assays with sorvent silica and elution 10 mL of the acetonitrile in three fortification levels. Recovery results for method were between 45-56%, 46-64%, 49-64%, 34-43% for the pesticides pirimicarb, malathion, procymidone and bifenthrin, respectively, witch relative standard deviations lower than 8,5%. Limits of detection and quantification for the analytes investigated ranged from 0,02 0,088 μgmL-1 and 0,07 0,282 μgmL-1, respectively.