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Document Type: Artigo
Title: Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals
Authors: Luna, Tânia
Santos, Silvane Braga
Nascimento, Marcia Cristina Furtado
Porto, M. Aurélia Fonseca
Santos, André Luiz Muniz Alves dos
Carvalho Filho, Edgar Marcelino de
Jesus, Amélia Maria Ribeiro de
Issue Date: Nov-2011
Abstract: Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline, forskolin, rolipram, and thalidomide to decrease in vitro production of TNF-α and IFN-γ in cells of HTLV-1-infected subjects. Participants of the study included 19 patients with HAM/TSP (mean age, 53 ± 11; male:female ratio, 1:1) and 18 HTLV-1 carriers (mean age, 47 ± 11; male:female ratio, 1:2.6). Cytokines were determined by ELISA in supernatants of mononuclear cell cultures. Pentoxifylline inhibited TNF-α and IFN-γ synthesis with the minimum dose used (50 µM). The results with forskolin were similar to those observed with pentoxifylline. The doses of rolipram used were 0.01-1 µM and the best inhibition of TNF-α production was achieved with 1 µM and for IFN-γ production it was 0.01 µM. The minimum dose of thalidomide used (1 µM) inhibited TNF-α production but thalidomide did not inhibit IFN-γ production even when the maximum dose (50 µM) was used. All drugs had an in vitro inhibitory effect on TNF-α production and, with the exception of thalidomide, all of them also decreased IFN-γ production.
Keywords: HTLV-1
Paraparesia espástica tropical
Linfócito T
Necrose tumoral alfa
ISSN: 1414-431X
Publisher / Institution : Brazilian Journal of Medical and Biological Research
Citation: LUNA, T. et al. Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 44, n. 11, nov. 2011. Disponível em: <>. Acesso em: 20 fev. 2014.
License: Creative Commons Attribution License
Appears in Collections:DME - Artigos publicados em periódicos

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