Influência de RIP2 na densidade de volume ósseo do processo de cicatrização alveolar em camundongos

Abstract

The proper development of the bone repair process is fundamental for the restoration of tissue homeostasis and the recovery of its functions, or to enable rehabilitation therapies. Among the possible strategies for the therapeutic modulation of alveolar bone repair, we can highlight the modulation of the immune/inflammatory response, wich is extremely importante to the bone repais process, but whose functions are not yet clearly understood. RIP2 is a molecule that acts in the initialization of the immune/inflammatory response, through intracellular signaling after the activation of Nod like receptors (NLRs), either due to an infectious process or through some tissue damage. Since the trigger mechanisms of inflammatory mediators are not clear in the context of bone repair, we aim to characterize the role of RIP2 in the bone repair kinetics subsequent to extration of the upper incisor from mice. For this purpose, we use 16 genetically deficiente animals of the RIP2 signaling molecule (RIP2KO) and 16 animals wildtype (WT). 2 animals from each group were assigned for analysis by computerized microtomography (MCt) in the periods of 7 and 14 days after the surgical procedure, and another 3 animals for histomorphometric analysis, for each of the groups and periods (0 hora, 7, 14 e 21 dias). After the incisor extraction, the animals were sacrificed in the determined periods, and the samples were collected and prepared for analysis. The specimens were scanned, and the images were reconstructed and realigned. The samples targeted for histomorphometric analysis were stained with HE, and analyzed with the aid of a binocular optical microscope, for counting of coincident points and later calculation of the volume density. Through the MCt analysis, it was observed that the absence of RIP2 does not interfere with the bone formation kinetics after extraction. Although through the quantitative analysis of bone tissue, it was possible to observe some variations in the relation to the tissue and its components. The difference in the volume density of osteoblasts between the groups, in the periods of 14 and 21 days, drew attention due to a significant superiority of the RIP2KO group, wich generated hypotheses about the possible delay in the maturation of bone tissue in the absence of RIP2 molecule.