Please use this identifier to cite or link to this item: https://ri.ufs.br/jspui/handle/riufs/1601
Document Type: Artigo
Title: Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
Authors: Marques, Maria Elizabeth da Costa
Freitas, Beatriz Tupinambá
Guedes, Tarciana Oliveira
Fernandes, Roberta Pereira Miranda
Issue Date: Dec-2013
Abstract: The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively.
Keywords: Castor plant
Cytokinin dehydrogenase
Biochemical characterization
Peroxidase
Mamona
Citoquinas
Bioquímica
ISSN: 1516-8913
Publisher / Institution : Instituto de Tecnologia do Paraná (Tecpar)
Citation: MARQUES, M. E. C. et al. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, nov./dez. 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600003&lng=en&nrm=iso>. Acesso em: 26 jan. 2016.
License: Creative Commons Attribution License
URI: https://ri.ufs.br/handle/riufs/1601
Appears in Collections:DFS - Artigos de periódicos

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