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https://ri.ufs.br/jspui/handle/riufs/25068| Tipo de Documento: | Artigo |
| Título: | B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization |
| Autor(es): | Fontoura, Isabela Cardoso Trombone, Ana Paula Favaro Almeida, Luciana Previato de Lorenzi, Julio Cesar Cetrulo Rossetti, Renata Ariza Marques Malardo, Thiago Padilha, Everton Schluchting, William Roberto Silva, Ricardo Luís Louzada da Gembre, Ana Flávia Fiuza, José Eduardo Cury Silva, Celio Lopes Panunto-Castelo, Ademilson Coelho-Castelo, Arlete Aparecida Martins |
| Data do documento: | 2015 |
| Resumo: | In DNA vaccines, the gene of interest is cloned into a bacterial plasmid that is engineered to induce protein production for long periods in eukaryotic cells. Previous research has shown that the intramuscular immunization of BALB/c mice with a naked plasmid DNA fragment encoding the Mycobacterium leprae 65-kDa heat-shock protein (pcDNA3-Hsp65) induces protection against M. tuberculosis challenge. A key stage in the protective immune response after immunization is the generation of memory T cells. Previously, we have shown that B cells capture plasmid DNA-Hsp65 and thereby modulate the formation of CD8+ memory T cells after M. tuberculosis challenge in mice. Therefore, clarifying how B cells act as part of the protective immune response after DNA immunization is important for the development of more-effective vaccines. The aim of this study was to investigate the mechanisms by which B cells modulate memory T cells after DNA-Hsp65 immunization. C57BL/6 and BKO mice were injected three times, at 15-day intervals, with 100 mg naked pcDNA-Hsp65 per mouse. Thirty days after immunization, the percentages of effector memory T (TEM) cells (CD4+ and CD8+/CD44high/CD62Llow) and memory CD8+ T cells (CD8+/CD44high/CD62Llow/CD127+) were measured with flow cytometry. Interferon g, interleukin 12 (IL-12), and IL-10 mRNAs were also quantified in whole spleen cells and purified B cells (CD43– ) with real-time qPCR. Our data suggest that a B-cell subpopulation expressing IL-10 downregulated proinflammatory cytokine expression in the spleen, increasing the survival of CD4+ TEM cells and CD8+ TEM/CD127+ cells. |
| Palavras-chave: | DNA-Hsp65 vaccine Memory T cells B cells |
| ISSN: | 1414-431X |
| Parte de : | Brazilian Journal of Medical and Biological Research |
| Idioma: | eng |
| Instituição/Editora: | Associação Brasileira de Divulgação Científica |
| Citação: | FONTOURA, I. C. et al. B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 12, p. 1095–1100, 2015. Disponível em: https://www.scielo.br/j/bjmbr/a/ZQMMVKbsG4CnF98hMb4XtdP/?lang=en. Acesso em: 1 abr. 2026. |
| Licença: | Creative Commons Atribuição 4.0 Internacional (CC BY 4.0) |
| Identificador: | https://doi.org/10.1590/1414-431X20154409 |
| URI: | https://ri.ufs.br/jspui/handle/riufs/25068 |
| Aparece nas coleções: | DESL - Artigos de periódicos |
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| BCellsExpressingIL-10mRNAModulateMemory.pdf | 275,09 kB | Adobe PDF |  Visualizar/Abrir |
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