Please use this identifier to cite or link to this item:
https://ri.ufs.br/jspui/handle/riufs/865| Document Type: | Artigo |
| Title: | Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1 |
| Authors: | Cândido, Alexandre Luna Bontempo, Elizabete Dias Resende, Maurício |
| Issue Date: | Apr-2010 |
| Abstract: | Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification. |
| Keywords: | Herpesvirus bovino Co-solventes DNA Reação em cadeia da polimerase |
| ISSN: | 0074-0276 |
| Publisher / Institution : | Fundação Oswaldo Cruz |
| Citation: | CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014. |
| License: | Creative Commons Attribution License |
| URI: | https://ri.ufs.br/handle/riufs/865 |
| Appears in Collections: | DMO - Artigos de periódicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| PolymeraseChainReaction.pdf | 45,26 kB | Adobe PDF |  View/Open |
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