Please use this identifier to cite or link to this item: http://ri.ufs.br/jspui/handle/riufs/865
Document Type: Artigo
Title: Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
Authors: Cândido, Alexandre Luna
Bontempo, Elizabete Dias
Resende, Maurício
Issue Date: Apr-2010
Abstract: Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
Keywords: Herpesvirus bovino
Co-solventes
DNA
Reação em cadeia da polimerase
ISSN: 0074-0276
Publisher / Institution : Fundação Oswaldo Cruz
Citation: CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
License: Creative Commons Attribution License
URI: https://ri.ufs.br/handle/riufs/865
Appears in Collections:DMO - Artigos publicados em periódicos

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