Efeito anti-inflamatório e antioxidante da gliciteína em modelo de edema de orelha em camundongos

Abstract

Inflammation is a protective factor for the body, but it can cause tissue damage when excessive. Considering inflammatory skin diseases and the limitations of their conventional treatments, it is interesting to develop therapies that can be administered topically. In this sense, glycitein, a soy isoflavone, may be a promising alternative. The present study investigated the anti-inflammatory and antioxidant effects of glycitein in a mouse model of acute skin inflammation. The study was approved by the Animal Use Ethics Committee (CEUA) of the Federal University of Sergipe under protocol number 8947220923. The animals received a topical administration of TPA (12-O tetradecanoylphorbol 13-acetate; 1 μg/ear) in the right ear to induce inflammation. 5 min later, they were treated with glycitein (0.3, 1.0, and 3.0 mg/ear), vehicle (acetone), or dexamethasone (0.05 mg/ear). Euthanasia and removal of the ears occurred 6 h later. Ear analyses showed that glycitein reduced ear edema (24.2% p=0.036; 36.8% p=0.0002 and 33.2% p=0.001), myeloperoxidase activity (90.1, 87.2 and 72.1%, p<0.0001 each) and tissue concentrations of IL-6 (58.5% p=0.0007; 56.5% p=0.0006; 66.8% p<0.0001), IL-1β (57.3% p=0.0002; 54.8% p=0.0002; 59.3% p<0.0001) and TNF-α (54.9% p=0.011; 61.2% p=0.002; 61.6% p=0.002), for 0.3, 1.0 and 3.0 mg/ear respectively compared to the vehicle group. In the histological evaluation, less intense edema and dermo-epidermal thickness were observed in the treatment with doses of 1.0 and 3.0 mg/ear (p<0.0001, each), but not for 0.3 mg/ear, in relation to the control. In addition, treatment with glycitein decreased the concentrations of total hydroperoxides by 60.1% (p=0.014) and 81.4% (p=0.0004) for 1.0 and 3.0 mg/ear, respectively. Furthermore, the dose of 1.0 mg/ear was tested for antioxidant activity and promoted a increase of 55.8% (p=0.009) for catalase (CAT) activity and 76.3% (p=0.001) for superoxide dismutase (SOD). These results demonstrate that glycitein has anti-inflammatory and antioxidant effects in the TPA-induced ear edema model in mice and has the potential to contribute to the development of new topical treatments for inflammatory skin diseases.