Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.

Abstract

The complexity of compounds present in plants allows them to be used for different diseases, which is due to the presence of bioactive compounds named as secondary metabolites. Considering the lack of chemical knowledge of Croton grewioides species and the need to establish new methodologies for the extraction of its antioxidante compounds, in this work was carried out the optimization of the extraction processes by ultrasound and microwave using an experimental planning, aided by statistical analysis. Thus, it was possible to determine the best extraction conditions for each of the technique studied, ultrasound-assisted extraction (UAE) and microwave assisted extraction (MAE). From the best conditions of extraction, the extracts of C. grewioides were analyzed by liquid chromatography and their fingerprint chromatograms submitted to chemometric analysis. High resolution mass spectrometry analyzes were also carried out, using the Orbitrap analyzer, with the objective of obtaining a chemical profile of mass spectrums, chemical profile of the extracts, and try to chemically identify the molecular formula and propose the chemical structure to the largest number possible of compounds present in this species. Through the chemometric results from both fingerprint, the identified metabolites and the antioxidant activities obtained, it was possible to assigned that the best extraction technique for antioxidant compounds from C. grewioides is microwave assisted extraction. Additionally, it was possible to propose, for the first time, structures of forty one compounds in the extracts of this species from the analysis of high resolution mass spectrometry, being considered an unprecedented method for the chemical study of this species.